1. A review on magnetic beads-based SELEX technologies: Applications from small to large target molecules.
- Author
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Manea, Ioana, Casian, Magdolna, Hosu-Stancioiu, Oana, de-los-Santos-Álvarez, Noemí, Lobo-Castañón, María Jesús, and Cristea, Cecilia
- Subjects
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SMALL molecules , *XENOBIOTICS , *MOLECULES , *MICROFLUIDICS , *APTAMERS , *TARGETED drug delivery , *TOXINS , *HERBICIDES - Abstract
This review summarizes the stepwise strategy and key points for magnetic beads (MBs)-based aptamer selection which is suitable for isolating aptamers against small and large molecules via systematic evolution of ligands by exponential enrichment (SELEX). Particularities, if any, are discussed according to the target size. Examples targeting small molecules (<1000 Da) such as xenobiotics, toxins, pesticides, herbicides, illegal additives, hormones, and large targets such as proteins (biomarkers, pathogens) are discussed and presented in tabular formats. Of special interest are the latest advances in more efficient alternatives, which are based on novel instrumentation, materials or microelectronics, such as fluorescence MBs-SELEX or microfluidic chip system-assisted MBs-SELEX. Limitations and perspectives of MBs-SELEX are also reviewed. Taken together, this review aims to provide practical insights into MBs-SELEX technologies and their ability to screen multiple potential aptamers against targets from small to large molecules. [Display omitted] • Principle and key points of magnetic beads-based SELEX technologies are summarized. • Particularities of aptamer screening from small to large molecules are provided. • Post-SELEX strategies to manipulate the performance of aptamers are introduced. • Aptamers for biomedical, food and environmental applications are reviewed. • Advantages and limitations of MBs-based screening tools are outlined. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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