1. Bio-barcode assay: A useful technology for ultrasensitive and logic-controlled specific detection in food safety: A review.
- Author
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Hou, Yue, Chen, Ruipeng, Wang, Zhiguang, Lu, Ran, Wang, Yonghui, Ren, Shuyue, Li, Shuang, Wang, Yu, Han, Tie, Yang, Shiping, Zhou, Huanying, and Gao, Zhixian
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FOOD safety , *DNA probes , *FOOD testing , *GENE amplification , *PATHOGENIC bacteria , *ORGANOPHOSPHORUS pesticides , *PESTICIDES , *PESTICIDE residues in food - Abstract
Food safety is one of the greatest public health challenges. Developing ultrasensitive detection methods for analytes at ultra-trace levels is, therefore, essential. In recent years, the bio-barcode assay (BCA) has emerged as an effective ultrasensitive detection strategy that is based on the indirect amplification of various DNA probes. This review systematically summarizes the progress of fluorescence, PCR, and colorimetry-based BCA methods for the detection of various contaminants, including pathogenic bacteria, toxins, pesticides, antibiotics, and other chemical substances in food in over 120 research papers. Current challenges, including long experimental times and strict storage conditions, and the prospects for the application of BCA in biomedicine and environmental analyses, have also been discussed herein. [Display omitted] • Bio-barcode assay (BCA) is a novel rapid detection technology. • BCA has several advantages over conventional methods. • We summarize the concept and detection principles of BCA. • BCA has extensive applications in food testing. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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