1. A two-photon fluorescent turn-on probe for imaging of SO2 derivatives in living cells and tissues.
- Author
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Zhu, Xiaoyan, Zhu, Longming, Liu, Hong-wen, Hu, Xiaoxiao, Peng, Rui-zi, Zhang, Jing, Zhang, Xiao-Bing, and Tan, Weihong
- Subjects
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CELL imaging , *PHOTONS , *FLUORESCENCE , *SULFUR dioxide , *CELL physiology - Abstract
SO 2 and its derivatives (bisulfite/sulfite) play crucial roles in several physiological processes. Therefore, development of reliable analytical methods for monitoring SO 2 and its derivatives in biological systems is very significant. In this paper, a FRET-based two-photon fluorescent turn-on probe, A-HCy , was proposed for specific detection of SO 2 derivatives through the bisulfite/sulfite-promoted Michael addition reaction. In this FRET system, an acedan (2-acetyl-6-dialkylaminonaphthalene) moiety was selected as a two-photon donor and a hemicyanine derivative served as both the quencher and the recognition unit for bisulfite/sulfite. A-HCy exhibited excellent selectivity and rapid response to HSO 3 − with a detection limit of 0.24 μM. More importantly, probe A-HCy was first successfully applied in two-photon fluorescence imaging of biological SO 2 derivatives in living cells and tissues, suggesting its great potential for practical application in biological systems. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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