Search

The analysis of trace hydrophilic targets in complex aqueous-rich matrices is considerably challenging, generally requiring matrix-matched calibration, internal standard, or time-and-labor-intensive sample preparation. To address this analytical bottleneck, a non-matrix-matched calibration strategy without using internal standard was reported for the first time to analyze complicated biosamples such as whole blood, plasma, serum, and cell samples. This strategy, termed micelle-dominated distribution, also aimed at realizing the simple "extract-and-shoot" analytical process for such complex matrices. The micelle-matrix interaction was found to efficiently eliminate the matrix effect by dominating phase separation and analyte distribution between the extraction and matrix phases. Thus, calibration linear curves prepared in water were applicable to the analysis of all the above-mentioned sample types. Rapid distribution equilibrium within 4 min was achieved. This strategy could tolerate direct large volume injection, thereby providing two-order-of-magnitude enhancement in the sensitivity of ion-pair chromatography. The analytical method integrated cell rupture, matrix cleanup, analyte extraction, and on-column preconcentration into a fast and high-throughput operation. The successful application to the determination of exogenous pesticides and endogenous glutathione exhibited low limits of detection (0.0085-0.015 μg mL -1 for pesticides; 0.52 μg mL -1 for glutathione), wide linear ranges (0.028-50 μg mL -1 and 0.049-50 μg mL -1 for pesticides; 1.7-1000 μg mL -1 for glutathione), good linearies (R 2  = 0.9994-0.9999), excellent accuracy (recoveries of 91.3-105.2%), and good precision (0.7-6.2% at the levels of 0.028 (or 0.049), 0.1, 0.5, and 50 μg mL -1 for pesticides; 0.5-8.7% at 1.7, 500, and 1000 μg mL -1 for glutathione)., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2019 Elsevier B.V. All rights reserved.)

CdSe/ZnS QDs have demonstrated capacity to act as reducing agent in organic media such as acetonitrile and ethanol. By using fluorescence and Raman spectroscopy, it has been demonstrated that QDs reduce diquat herbicide to its monocation radical. The reaction is characterized to present a high reaction rate making possible to perform the reaction by simple filtration of the solution containing the herbicide through a QDs modified filter. The monocation radical presents a high fluorescence emission spectrum which was selected as the analytical signal to quantify the diquat herbicide. The method described here for the analysis of diquat herbicide in oat grains is simple and fast allowing the analysis of trace level of herbicide in only 6 min. The excellent sensitivity and reproducibility of the methods indicate that the reaction is favoured from both thermodynamic and kinetic point of view. The results presented open up the possibility to use QDs as redox agent. The sensitivity of the method expressed as detection limit was only of 0.01 mg kg(-1).The lineal range was between 0.05 and 0.5 mg kg(-1). The time of analysis per sample, including extraction, reaction and fluorescent measurement was only of 6 min., (Copyright © 2011 Elsevier B.V. All rights reserved.)