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Functionalized micro/nanomotors having immobilized biological molecules provide excellent and powerful tools for the detection of target molecules. Based on surface modifications and mobilities of micromotors, we report herein a new experimental design of high-speed, self-propelled and plasma modified micromotors for biomedical applications. Within this scope, in the first step, poly (3,4-ethylenedioxythiophene) (PEDOT) was in-situ synthesized onto W 5 O 14 (tungsten trioxide) wires by using radio frequency (RF) rotating plasma reactor. Then, W 5 O 14 /PEDOT-Platinum (Pt) hybrid micromotors were fabricated by using magnetron sputtering technique. The detection of miRNA-21 was performed using both single-stranded DNA (ssDNA) (probe DNA) immobilized W 5 O 14 -Pt and W 5 O 14 /PEDOT-Pt micromotors. The fluorescence signals were determined after hybridization of probe DNA immobilized these novel W 5 O 14 -Pt and W 5 O 14 /PEDOT-Pt micromotors with different molar concentrations of the synthetic target (6-carboxyfluorescein dye (FAM)-labeled miRNA-21). The changes in the micromotor speeds after the hybridization process were also evaluated. W 5 O 14 /PEDOT-Pt micromotors presented better sensor properties compared to the W 5 O 14 -Pt micromotors. A good linearity for miRNA-21 concentration between 0.1 nM and 100 nM was obtained for these micromotors based on their fluorescence intensities. The detection limit was found as 0.028 nM for W 5 O 14 /PEDOT-Pt micromotors (n = 3). Thus, sensor and motor characteristics of the W 5 O 14 -Pt micromotors were improved by RF plasma enhanced PEDOT coatings. The new catalytic W 5 O 14 based micromotors demonstrated here had great potential for the development of sensitive and simple sensing platforms for detection of miRNA-21., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier B.V. All rights reserved.)