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Fluorescence single-molecule spectroscopy is an appropriate tool for modern bioanalysis. This technique enables the development of ultra sensitive assays, especially when combined with self-quenching probes. In this review we report novel DNA, enzyme, and antibody assays based on mono-labeled fluorescent probes that are quenched by photoinduced electron transfer (PET). [ABSTRACT FROM AUTHOR]

The article reflects that, spectral fluorescence imaging microscopy is an appropriate method for imaging single molecules in living cells. Many methods have been proposed for analyzing single cells, over the last decade. However, some optical methods like fluorescence spectroscopy, are advanced and simple. The article also presents that because of their high sensitivity, spectrally resolved fluorescence lifetime imaging microscopy (SFLIM) requires only a few molecules to be probed.