1. Identification of human hepatocellular carcinoma-related proteins by proteomic approaches.
- Author
-
Ming-Hui Yang, Yu-Chang Tyan, Shiang-Bin Jong, Ying-Fong Huang, Pao-Chi Liao, and Ming-Chen Wang
- Subjects
LIVER tumors ,SERUM ,PROTEOMICS ,LIVER cancer ,HIGH performance liquid chromatography - Abstract
Hepatocellular carcinoma (HCC) is the most common malignant liver tumor. Analysis of human serum from HCC patients using two-dimensional gel electrophoresis (2DE) combined with nano-high-performance liquid chromatography electrospray ionization tandem mass spectrometry (nano-HPLC–ESI-MS/MS) identified fourteen different proteins differentially expressed between HCC patients and the control group. Twelve proteins were up-regulated and two down-regulated. By using nano-HPLC–MS/MS system to analyze proteome in human serum, 317 proteins were identified, twenty-nine of which to high confidence levels (protein matched at last two unique peptide sequences). Of these twenty-nine proteins, six were present only in HCC patients and may serve as biomarkers for HCC. [ABSTRACT FROM AUTHOR]
- Published
- 2007
- Full Text
- View/download PDF