1. Optical sensing in microfluidic lab-on-a-chip by femtosecond-laser-written waveguides
- Author
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Roberto Osellame, Rebeca Martínez Vázquez, Marcella Chiari, Giulio Cerullo, Hugo Hoekstra, C. Dongre, Roberta Ramponi, Hans H. van den Vlekkert, Markus Pollnau, and Marina Cretich
- Subjects
Optics and Photonics ,business.industry ,Chemistry ,Microfluidics ,Fluorescence spectrometry ,Physics::Optics ,Lab-on-a-chip ,Laser ,Biochemistry ,Waveguide (optics) ,Fluorescence ,Analytical Chemistry ,Numerical aperture ,law.invention ,Optics ,law ,IOMS-SNS: SENSORS ,Femtosecond ,Photonics ,business - Abstract
We use direct femtosecond laser writing to integrate optical waveguides into a commercial fused silica capillary electrophoresis chip. High-quality waveguides crossing the microfluidic channels are fabricated and used to optically address, with high spatial selectivity, their content. Fluorescence from the optically excited volume is efficiently collected at a 90 degree angle by a high numerical aperture fiber, resulting in a highly compact and portable device. To test the platform we performed electrophoresis and detection of a 23-mer oligonucleotide plug. Our approach is quite powerful because it allows the integration of photonic functionalities, by simple post-processing, into commercial LOCs fabricated with standard techniques.
- Published
- 2008
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