1. Development and application of a visualization method for identification of Panax species with LAMP and a DNAzyme.
- Author
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Wang, Xiangjun, Liu, Moyi, Li, Ying, Zhou, Xinchen, Zhang, Zhuo, Dong, Shuhan, Shen, Mingmei, Wang, Minghui, Wang, Helin, and Liu, Limei
- Subjects
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GINSENG , *DEOXYRIBOZYMES , *AMERICAN ginseng , *PANAX , *ADULTERATIONS , *LAMPS , *DATA visualization - Abstract
Panax ginseng and Panax quinquefolium are two valuable Chinese herbal medicines that should not be mixed because they differ in drug properties and efficacy. The traditional identification method is easily affected by subjective factors and cannot effectively distinguish between ginseng products. This study aimed to develop a new chemical analysis method to visually identify P. ginseng and P. quinquefolium. In this method, a large number of sequences containing G-quadruplex were generated by loop-mediated isothermal amplification, and the combination of G-quadruplex and hemin was used to form deoxyribozyme, which catalyzed the color change of H 2 O 2. Artificial simulation of adulteration experiments revealed that this method could detect more than 20% adulterated P. quinquefolium. Compared with the traditional identification methods, this technology was simpler and more efficient, providing a reference for developing rapid visual identification methods and reagents for P. ginseng and P. quinquefolium. [Display omitted] • Find the difference region between Panax ginseng and Panax quinquefolium and establish LAMP reaction. • To facilitate the observation of LAMP reaction results, G-4 complementary sequence was introduced into BIP sequence. • The visual detection method based on LAMP and DNAzyme can be used to identify the components of ginseng products. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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