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1. A filtration assay for solubilized prolactin receptors using polyethyleneimine-treated membrane filters.

Author

el-Hamzawy MA and Costlow ME

Subjects
Animals, Detergents pharmacology, Female, Filtration methods, Hydrogen-Ion Concentration, Liver analysis, Membranes, Artificial, Rats, Rats, Inbred Strains, Solubility, Polyethyleneimine, Polyethylenes, Receptors, Prolactin isolation & purification
Abstract

Free 125I-labeled ovine prolactin can be separated from detergent-solubilized prolactin-receptor complex by filtration on triacetate membrane filters pretreated with polyethyleneimine. Up to 98% of the total 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate solubilized prolactin-receptor complexes from rat liver bound to polyethyleneimine-treated membranes. This simple and rapid technique can be used to quantitate solubilized prolactin-receptor complexes.

Published
1988
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