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Start Over Descriptor "Immunomagnetic capture" Journal analytical biochemistry
2 results on '"Immunomagnetic capture"'

1. Immunomagnetic capture and colorimetric detection of malarial biomarker Plasmodium falciparum lactate dehydrogenase.

Author

Markwalter, Christine F., Davis, Keersten M., and Wright, David W.

Subjects
*PLASMODIUM falciparum, *MALARIA, *BIOMARKERS, *LACTATE dehydrogenase, *MAGNETIC particles, *COLORIMETRIC analysis, *ENZYME-linked immunosorbent assay
Abstract

We report a sensitive, magnetic bead-based colorimetric assay for Plasmodium falciparum lactate dehydrogenase ( Pf LDH) in which the biomarker is extracted from parasitized whole blood and purified based on antigen binding to antibody-functionalized magnetic particles. Antigen-bound particles are washed, and Pf LDH activity is measured on-bead using an optimized colorimetric enzyme reaction (limit of detection [LOD] = 21.1 ± 0.4 parasites/μl). Enhanced analytical sensitivity is achieved by removal of Pf LDH from the sample matrix before detection and elimination of nonspecific reductases and species that interfere with the optimal detection wavelength for measuring assay development. The optimized assay represents a simple and effective diagnostic strategy for P. falciparum malaria with time-to-result of 45 min and detection limits similar to those of commercial enzyme-linked immunosorbent assay (ELISA) kits, which can take 4–6 h. This method could be expanded to detect all species of malaria by switching the capture antibody on the magnetic particles to a pan-specific Plasmodium LDH antibody. [ABSTRACT FROM AUTHOR]

Published
2016
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2. Immunomagnetic capture and colorimetric detection of malarial biomarker Plasmodium falciparum lactate dehydrogenase

Author

Keersten M. Davis, Christine F. Markwalter, and David W. Wright

Subjects
Immunomagnetic capture, Plasmodium falciparum, Biophysics, 02 engineering and technology, Immunomagnetic separation, 01 natural sciences, Biochemistry, Colorimetry (chemical method), chemistry.chemical_compound, Limit of Detection, Lactate dehydrogenase, parasitic diseases, Humans, Activity assay, Malaria, Falciparum, Molecular Biology, Whole blood, Enzyme Assays, Detection limit, Chromatography, biology, L-Lactate Dehydrogenase, Immunomagnetic Separation, Plasmodium lactate dehydrogenase, 010401 analytical chemistry, Cell Biology, 021001 nanoscience & nanotechnology, biology.organism_classification, Molecular biology, Enzyme assay, Malaria, 0104 chemical sciences, 3. Good health, chemistry, biology.protein, Colorimetry, Antibody, 0210 nano-technology
Abstract

We report a sensitive, magnetic bead-based colorimetric assay for Plasmodium falciparum lactate dehydrogenase (PfLDH) in which the biomarker is extracted from parasitized whole blood and purified based on antigen binding to antibody-functionalized magnetic particles. Antigen-bound particles are washed, and PfLDH activity is measured on-bead using an optimized colorimetric enzyme reaction (limit of detection [LOD] = 21.1 ± 0.4 parasites/μl). Enhanced analytical sensitivity is achieved by removal of PfLDH from the sample matrix before detection and elimination of nonspecific reductases and species that interfere with the optimal detection wavelength for measuring assay development. The optimized assay represents a simple and effective diagnostic strategy for P. falciparum malaria with time-to-result of 45 min and detection limits similar to those of commercial enzyme-linked immunosorbent assay (ELISA) kits, which can take 4–6 h. This method could be expanded to detect all species of malaria by switching the capture antibody on the magnetic particles to a pan-specific Plasmodium LDH antibody.

Published
2015

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