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Start Over Descriptor "J-RESOLVED SPECTROSCOPY" Journal analytical biochemistry
2 results on '"J-RESOLVED SPECTROSCOPY"'

1. Isotopic profiling of 13C-labeled biological samples by two-dimensional heteronuclear J-resolved nuclear magnetic resonance spectroscopy

Author

Cahoreau, Edern, Peyriga, Lindsay, Hubert, Jane, Bringaud, Frédéric, Massou, Stéphane, and Portais, Jean-Charles

Subjects
*NUCLEAR magnetic resonance spectroscopy, *CARBON isotopes, *ANALYTICAL chemistry, *METABOLIC flux analysis, *DATA analysis, *NUCLEAR spectroscopy
Abstract

Abstract: The use of two-dimensional heteronuclear J-resolved (2D H-JRES) nuclear magnetic resonance (NMR) spectroscopy for fast and reliable measurement of isotopic patterns from 13C-enriched compounds resulting from carbon labeling experiments was evaluated. Its use with biological samples of increasing complexity showed that 2D H-JRES spectroscopy is suitable for high-throughput isotopic profiling of any kind of labeled samples. Moreover, the method enabled accurate quantification of 13C enrichments and, thus, can be used for metabolic flux analysis. The excellent trade-off between reduced experimental time and the number of measurable isotopic data makes 2D H-JRES NMR a promising approach for high-throughput flux analysis of samples of intermediate complexity. [Copyright &y& Elsevier]

Published
2012
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2. Isotopic profiling of 13C-labeled biological samples by two-dimensional heteronuclear J-resolved nuclear magnetic resonance spectroscopy

Author

Edern Cahoreau, Jane Hubert, Stéphane Massou, Frédéric Bringaud, Jean-Charles Portais, Lindsay Peyriga, Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées, Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP), Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Recherche Agronomique (INRA), Centre de résonance magnétique des systèmes biologiques (CRMSB), Centre National de la Recherche Scientifique (CNRS)-Université de Bordeaux (UB), Résonance magnétique des systèmes biologiques (RMSB), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS), Université de Toulouse (UT), Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), MetaToul FluxoMet (TBI-MetaToul), MetaboHUB-MetaToul, MetaboHUB-Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Université de Toulouse (UT)-Université de Toulouse (UT)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-MetaboHUB-Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Toulouse Biotechnology Institute (TBI), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS)

Subjects
C-13, [SDV]Life Sciences [q-bio], Biophysics, Analytical chemistry, H-1 NMR, 010402 general chemistry, Mass spectrometry, 01 natural sciences, Biochemistry, J-resolved spectroscopy, GLUTAMATE, 03 medical and health sciences, Nuclear magnetic resonance, Metabolic flux analysis, 2D NMR, METABOLIC FLUX ANALYSIS, Spectroscopy, Molecular Biology, 030304 developmental biology, J resolved, 0303 health sciences, Isotopic profiling, STABILITY, Chemistry, MIXTURES, MASS-SPECTROMETRY, Cell Biology, Nuclear magnetic resonance spectroscopy, QUANTITATIVE-ANALYSIS, 0104 chemical sciences, Intermediate complexity, Heteronuclear molecule, ESCHERICHIA-COLI, NMR-SPECTROSCOPY, C-13 enrichment, Two-dimensional nuclear magnetic resonance spectroscopy, Carbon labeling experiment, LABELING EXPERIMENTS
Abstract

The use of two-dimensional heteronuclear J-resolved (2D H-JRES) nuclear magnetic resonance (NMR) spectroscopy for fast and reliable measurement of isotopic patterns from C-13-enriched compounds resulting from carbon labeling experiments was evaluated. Its use with biological samples of increasing complexity showed that 2D H-JRES spectroscopy is suitable for high-throughput isotopic profiling of any kind of labeled samples. Moreover, the method enabled accurate quantification of C-13 enrichments and, thus, can be used for metabolic flux analysis. The excellent trade-off between reduced experimental time and the number of measurable isotopic data makes 2D H-JRES NMR a promising approach for high-throughput flux analysis of samples of intermediate complexity. (C) 2012 Elsevier Inc. All rights reserved.

Published
2012
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