1. Previsible silver staining of protein in electrophoresis gels with mass spectrometry compatibility
- Author
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Wei-Tao Cong, Xiaokun Li, Sun-Young Hwang, Litai Jin, and Jung-Kap Choi
- Subjects
Proteomics ,Silver Staining ,Time Factors ,Biophysics ,Sodium thiosulfate ,Mass spectrometry ,Biochemistry ,Sensitivity and Specificity ,Mass Spectrometry ,Silver stain ,chemistry.chemical_compound ,Rosaniline Dyes ,Animals ,Coloring Agents ,Molecular Biology ,Gel electrophoresis ,Detection limit ,Chromatography ,Silver Staining Method ,Analytic Sample Preparation Methods ,Proteins ,Cell Biology ,Staining ,Electrophoresis ,Zinc ,chemistry ,Costs and Cost Analysis ,Linear Models ,Cattle ,Electrophoresis, Polyacrylamide Gel - Abstract
A convenient silver staining method for protein in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels is described. The method is previsible, sensitive, and mass spectrometry (MS) compatible. Two visible counter ion dyes, ethyl violet (EV) and zincon (ZC), were used in the first staining solution with a detection limit of 2 to 8 ng/band in approximately 1h. The dye-stained gel can be further stained by silver staining, which is based on acidic silver staining employing ZC with sodium thiosulfate as silver ion sensitizers. Especially, ZC has silver ion reducing power by cleavage of the diazo bond of the dye during silver reduction. The second silver staining can be completed in approximately 1h with a detection limit of 0.2 ng/band. more...
- Published
- 2008