1. Tremella fuciformis Polysaccharides Attenuate Oxidative Stress and Inflammation in Macrophages through miR-155.
- Author
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Ruan, Yang, Li, Hong, Pu, Lianmei, Shen, Tao, and Jin, Zening
- Subjects
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OXIDATIVE stress , *MACROPHAGES , *POLYSACCHARIDES , *LIPOPOLYSACCHARIDES , *INFLAMMATION , *NON-coding RNA - Abstract
Aim. To investigate the function ofTremella fuciformis polysaccharides (TFPS) in LPS-induced inflammation and oxidative stress of macrophages.Methods . RAW264.7 cells were pretreated with TFPS and then stimulated with 0.1μ g/ml LPS. NFκ B, Akt, p38MAPK, MCP-1, and SOD-1 were analyzed by Western blotting. Cell viability was measured using MTT assays. Reactive oxygen species (ROS) production, real-time PCR, ELISA, and immunofluorescence staining were performed on RAW264.7 cells that were treated with LPS and/or TFPS to investigate the anti-inflammatory effect of TFPS.Results. LPS induced inflammation and ROS production and promoted the secretion of cytokines such as TNF-α and IL-6. LPS also enhanced the nuclear translocation of NFκ B, which promoted inflammation by oxidative stress. However, pretreatment with TFPS profoundly inhibited the activation of Akt, p38MAPK, and NFκ B and attenuated the expression of MCP-1 in macrophages. Meanwhile, TFPS also decreased cytokine and ROS levels and attenuated cell inflammation after treatment with LPS. Moreover, miR-155, one of the key small RNAs which regulate NFκ B and inflammation in macrophages, was significantly downregulated.Conclusion . TFPS inhibits LPS-induced oxidative stress and inflammation by inhibiting miR-155 expression and NFκ B activation in macrophages, which suggests that TFPS may be a potential reagent for inhibiting the development of inflammation. [ABSTRACT FROM AUTHOR]- Published
- 2018
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