1. Multiple Functional Nanoprobe for Contrast-Enhanced Bimodal Cellular Imaging and Targeted Therapy.
- Author
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Hong-Min Meng, Limin Lu, Xu-Hua Zhao, Zhuo Chen, Zilong Zhao, Chan Yang, Xiao-Bing Zhang, and Weihong Tan
- Subjects
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CELL imaging , *CANCER treatment , *NANO-probe sensors , *CANCER chemotherapy , *TWO-photon-spectroscopy - Abstract
Many one-photon fluorescence-based theranostic nanosystems have been developed for simultaneous therapeutic intervention/monitoring for various types of cancers. However, for early diagnosis of cancer, two-photon fluorescence microscopy (TPFM) can realize deep-tissue imaging with higher spatial resolution. In this study, we first report a multiple functional nanoprobe for contrast-enhanced bimodal cellular imaging and targeted therapy. Components of the nanoprobe include (1) two-photon dye-doped mesoporous silica nanoparticles (TPD-MSNs); (2) MnO2 nanosheets that act as a (i) gatekeeper for TPD-MSNs, (ii) quencher for TP fluorescence, and (iii) contrast agent for MRlj (3) cancer cell-targeting aptamers. Guided by aptamers, TPDMSNs are rapidly internalized into the target cells. Next, intracellular glutathione reduces Mn2 to Mn2+ ions, resulting in contrast-enhanced TP fluorescence and magnetic resonance signal for cellular imaging. Meanwhile, preloaded doxorubicin and Chlorin e6 are released for chemotherapy and photodynamic therapy, respectively, with a synergistic effect and significantly enhanced therapeutic efficacy. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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