1. Antioxidant redox sensors based on DNA modified carbon screen-printed electrodes.
- Author
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Liu J, Su B, Lagger G, Tacchini P, and Girault HH
- Subjects
- Metal Nanoparticles, Microscopy, Electron, Scanning, Oxidation-Reduction, Titanium chemistry, Antioxidants chemistry, Carbon chemistry, DNA chemistry, Electrodes
- Abstract
Antioxidant redox sensors based on DNA modified carbon screen-printed electrodes were developed. The carbon ink was doped with TiO2 nanoparticles, onto which double-strand DNA was adsorbed. A redox mediator, namely, tris-2,2'-bipyridine ruthenium(II) [Ru(bpy)3(2+)] was electrooxidized on the electrode surface to subsequently oxidize both the adsorbed ds-DNA and the antioxidants in solution. The resulting oxidation damage of the adsorbed ds-DNA was then detected by square wave voltammetry in a second solution containing only Ru(bpy)3Cl2 at a low concentration (microM). A kinetic model was developed to study the protecting role of antioxidants in aqueous solutions. The electrochemical sensor has been applied to evaluate the redox antioxidant capacity of different molecules.
- Published
- 2006
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