Search

Glutathione (GSH) serves vital functions in biological systems and associates with various human diseases. In this work, a versatile electrochemiluminence (ECL) and a photoelectrochemical (PEC) "signal on" biosensing platform were developed for a sensitive assay of GSH by a Mn2+-powered DNAzyme amplification strategy combined with DNA-walker-triggered allosteric conversion. First, MnO2 nanosheets were reduced to Mn2+ by GSH; then, Mn2+ as a substitute target triggered DNAzyme-assisted cleavage-cycling amplification to generate numerous DNA output (s3). Meanwhile, the DNA molecular machine was introduced to bridge signal probes for versatile biosensing, which included hairpin DNA as a track and an arm as a walker. The presence of DNA output (s3) activated the swing arm to hybridize with hairpin DNA and then cut it by Nt.BbvCI, which initiated autonomous walking of the arm for forming a large number of streptavidin (SA) aptamers. Thus, a large number of CdS:Mn-SA tags as versatile signal probes was linked to the electrode by specific SA-aptamer binding, generating highly enhanced ECL and PEC signals for sensitive detection of the target. The present biosensing system take advantage of metal ion-based DNAzyme amplification, a DNA walker machine, multi-signals of QDs, and specificity of aptamers, which can provide a universal and efficient biosensing method for detecting various targets. The designed strategy demonstrated good performance for a GSH assay in human serum samples, showing more promising applications than other reported methods.

Glutathione (GSH) serves vital functions in biological systems and associates with various human diseases. In this work, a versatile electrochemiluminence (ECL) and a photoelectrochemical (PEC) "signal on" biosensing platform were developed for a sensitive assay of GSH by a Mn

Herein, a new multifunctional DNA nanotube (DNANT) was self-assembled and used to load Ru(phen)32+ and methylene blue (MB) as amplified signal probes for versatile electrochemiluminescence (ECL) and electrochemical (EC) “on–off” assays of Dam methylase (MTase) and aflatoxin B1 (AFB1). The DNA nanotube as a carrier could immobilize numerous MB or Ru(phen)32+ species in the double-stranded DNA (dsDNA) to significantly amplify signals, which enabled highly sensitive ECL and electrochemical detection of dual targets. The target Dam MTase first catalyzed the methylation of hairpin DNA (H1), and then the methylated DNA was cleaved by endonuclease DpnI to expose a single-strand DNA. After the Ru(phen)32+-DNANT or MB-DNANT signal probes were assembled to the electrode by hybridization, remarkable “signal on” states for amplified ECL or EC assays of MTase were obtained. Furthermore, in the presence of the target AFB1, the structure of DNANTs collapsed due to the specific binding of AFB1 to aptamer S2 in NTs, which...