1. Sequencing of argentinated peptides by means of matrix-assisted laser desorption/ionization tandem mass spectrometry
- Author
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Chu, Ivan K., Cox, David M., Guo, Xu, Kireeva, Inga, Lau, Tai-Chu, McDermott, John C., and Siu, K.W. Michael
- Subjects
Peptides -- Analysis ,Mass spectrometry -- Methods ,Chemistry - Abstract
Argentinated peptide ions are formed in abundance under matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) conditions in the presence of [Ag.sup.+] ions. These argentinated peptide ions are fragmented facilely under MALDI-MS/MS conditions to yield [[[b.sub.n] + OH + Ag].sup.+], [[[b.sub.n] - H + Ag].sup.+] and [[[a.sub.n] - H + Ag].sup.+] ions that are indicative of the C-terminal sequence. These observations parallel those made earlier under electrospray MS conditions (Chu, I. K.; Guo, X.; Lau, T.-C.; Siu, K. W. M. Anal. Chem. 1999, 71, 2364-2372). A mixed protonated and argentinated tryptic peptide map was generated from 37 fmol of bovine serum albumin (BSA) using MALDI-MS. MALDI-MS/MS data from four argentinated peptides at a protein amount of 350 fmol unambiguously identified the protein as BSA. Sequence-tag analysis of two argentinated tryptic peptides was used to identify unambiguously myocyte enhancer factor 2A, which had been recombinantly expressed in a bacterial cell line.
- Published
- 2002