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Start Over Author "Liu, Jianbo" Journal analytical chemistry
23 results on '"Liu, Jianbo"'

4. Real-time imaging of protein internalization using aptamer conjugates

Author

Li, Wei, Yang, Xiaohai, Wang, Kemin, Tan, Weihong, He, Yan, Guo, Qiuping, Tang, Hongxing, and Liu, Jianbo

Subjects
Real-time control -- Research, Real-time systems -- Research, Imaging systems -- Methods, Proteins -- Properties, Angiogenin -- Properties, Peptides -- Properties, Real-time system, Chemistry
Abstract

Angiogenin is a potent angiogenic factor that is known to play an important role in tumor angiogenesis. In this paper, we investigate the cellular internalization of angiogenin conjugated with its highly specific aptamer. By using fluorophore-labeled aptamer and confocal laser scanning microscopy, we have developed a novel and simple method by which to visualize the real-time process of angiogenin internalization. Specifically, when aptamer--angiogenin conjugates were added into cell cultures, conjugates could be selectively bound to HUVE cells (human umbilical vein endothelial cells) and MCF-7 cells (human breast cancer cells). Nuclear staining and Z-axis scanning studies demonstrated that the aptamer--angiogenin conjugates were internalized to intracellular organelles, and dynamic confocal imaging studies indicated that the conjugates were quickly internalized. These results provide the first evidence that a fluorophore-labeled aptamer can be used as a fluorescent probe to visualize the spatiotemporal process of protein internalization in real time.

Published
2008

7. CuInS2/Red Phosphorus Nanosheet Interleaved Heterostructures with Improved Interfacial Charge Transfer for Photoelectrochemical Aptasensing

Author

Li, Yunpeng, Adili, Guliqire, Liang, Gang, Ma, Yuhua, and Liu, Jianbo

Abstract

Accelerating the migration of interfacial carriers in heterojunctions is crucial for achieving highly sensitive photoelectrochemical (PEC) sensing. In this study, we developed three-dimensional (3D)/two-dimensional (2D) CuInS2/red phosphorus nanosheet (CuInS2/RP NS) n–n heterojunction functional materials with enhanced interfacial charge transfer capabilities for PEC sensing. The 3D CuInS2serves as a conductive layer, providing excellent electronic conductivity and superior electron absorption and transport properties. In contrast, the ultrathin RP NS acts as a transport layer that enhances carrier mobility. The 3D/2D heterojunction ensures a large interface contact surface, shortening the carrier transport distance. A well-aligned band position generates a substantial built-in electric field, providing a significant driving force for efficient carrier separation and migration, thereby improving response sensitivity. A PEC aptamer sensor was constructed based on the synthesized heterostructure for ciprofloxacin detection. The detection limit of the CuInS2/RP NS aptamer sensor for ciprofloxacin is 2.03 × 10–15mg·mL–1, with a linear range from 1.0 × 10–14to 1.0 × 10–5mg·mL–1. This work presents a strategy for enhancing the photoelectric response by modulating the interface structure of heterojunctions, thereby opening new prospects for the application of highly sensitive PEC sensors in antibiotic detection.

Published
2024
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19. Single-Walled Carbon Nanotubes (SWCNTs)-Assisted Cell-Systematic Evolution of Ligands by Exponential Enrichment (Cell-SELEX) for Improving Screening Efficiency

Author

Tan, Yuyu, primary, Guo, Qiuping, additional, Xie, Qin, additional, Wang, Kemin, additional, Yuan, Baoyin, additional, Zhou, Yu, additional, Liu, Jianbo, additional, Huang, Jin, additional, He, Xiaoxiao, additional, Yang, Xiaohai, additional, He, Chunmei, additional, and Zhao, Xiayu, additional

Published
2014
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22. CuInS 2 /Red Phosphorus Nanosheet Interleaved Heterostructures with Improved Interfacial Charge Transfer for Photoelectrochemical Aptasensing.

Author

Li Y, Adili G, Liang G, Ma Y, and Liu J

Abstract

Accelerating the migration of interfacial carriers in heterojunctions is crucial for achieving highly sensitive photoelectrochemical (PEC) sensing. In this study, we developed three-dimensional (3D)/two-dimensional (2D) CuInS 2 /red phosphorus nanosheet (CuInS 2 /RP NS) n-n heterojunction functional materials with enhanced interfacial charge transfer capabilities for PEC sensing. The 3D CuInS 2 serves as a conductive layer, providing excellent electronic conductivity and superior electron absorption and transport properties. In contrast, the ultrathin RP NS acts as a transport layer that enhances carrier mobility. The 3D/2D heterojunction ensures a large interface contact surface, shortening the carrier transport distance. A well-aligned band position generates a substantial built-in electric field, providing a significant driving force for efficient carrier separation and migration, thereby improving response sensitivity. A PEC aptamer sensor was constructed based on the synthesized heterostructure for ciprofloxacin detection. The detection limit of the CuInS 2 /RP NS aptamer sensor for ciprofloxacin is 2.03 × 10 -15 mg·mL -1 , with a linear range from 1.0 × 10 -14 to 1.0 × 10 -5 mg·mL -1 . This work presents a strategy for enhancing the photoelectric response by modulating the interface structure of heterojunctions, thereby opening new prospects for the application of highly sensitive PEC sensors in antibiotic detection.

Published
2024
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23. Exciton energy transfer-based quantum dot fluorescence sensing array: "chemical noses" for discrimination of different nucleobases.

Author

Liu J, Li G, Yang X, Wang K, Li L, Liu W, Shi X, and Guo Y

Subjects
Fluorescence, Fluorescence Resonance Energy Transfer, Nucleosides analysis, Biosensing Techniques methods, Energy Transfer, Nanoparticles chemistry, Nucleosides chemistry, Quantum Dots
Abstract

A novel exciton energy transfer-based fluorescence sensing array for the discrimination of different nucleobases was developed through target nucleobase-triggered self-assembly of quantum dots (QDs). Four QD nanoprobes with different ligand receptors, including mercaptoethylamine, N-acetyl-l-cysteine, 2-dimethyl-aminethanethiol, and thioglycolic acid, were created to detect and identify nucleobase targets. These QDs served as both selective recognition scaffolds and signal transduction elements for a biomolecule target. The extent of particle assembly, induced by the analyte-triggered self-assembly of QDs, led to an exciton energy transfer effect between interparticles that gave a readily detectable fluorescence quenching and distinct fluorescence response patterns. These patterns are characteristic for each nucleobase and can be quantitatively differentiated by linear discriminate analysis. Furthermore, a fingerprint-based barcode was established to conveniently discriminate the nucleobases. This pattern sensing was successfully used to identify nucleobase samples at unknown concentrations and five rare bases. In this "chemical noses" strategy, the robust characteristics of QD nanoprobes, coupled with the diversity of surface functionality that can be readily obtained using nanoparticles, provides a simple and label-free biosensing approach that shows great promise for biomedical applications.

Published
2015
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