1. Efficient polymer monolith for strong cation-exchange capillary liquid chromatography of peptides.
- Author
-
Gu B, Chen Z, Thulin CD, and Lee ML
- Subjects
- Amino Acid Sequence, Buffers, Hydrogen-Ion Concentration, Microscopy, Electron, Scanning, Molecular Sequence Data, Proteins chemistry, Cations chemistry, Chromatography, Ion Exchange methods, Peptides chemistry, Peptides isolation & purification, Polymers chemistry
- Abstract
A stable poly[2-acrylamido-2-methyl-1-propanesulfonic acid-co-poly(ethylene glycol) diacrylate] monolith was synthesized inside a 75-microm-i.d. capillary by photoinitiated copolymerization with water, methanol, and ethyl ether as porogens. The resulting monolith was evaluated for strong cation-exchange capillary liquid chromatography of both synthetic and natural peptides. Although the monolith possessed relatively strong hydrophobicity due to the use of 2-acrylamido-2-methyl-1-propanesulfonic acid as one monomer, the monolith had a high dynamic binding capacity of 157 microequiv of peptide/mL, or 332 mg of cytochrome c/mL. Exceptionally high resolution resulting from extremely narrow peaks was obtained, resulting in a peak capacity of 179 when using a shallow salt elution gradient. Although a second, naturally formed gradient might contribute to the sharp peaks obtained, high efficiency was mainly due to the use of poly(ethylene glycol) diacrylate as a biocompatible cross-linker.
- Published
- 2006
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