1. General Sensor Design Strategy Based on G-Quadruplex-Hemin DNAzymes
- Author
-
De-Ming Kong, Li-Li Cai, Jing Xu, and Han-Xi Shen
- Subjects
Oligonucleotide ,Aptamer ,Biochemistry (medical) ,Clinical Biochemistry ,Deoxyribozyme ,G-quadruplex ,Biochemistry ,Combinatorial chemistry ,Redox ,Analytical Chemistry ,chemistry.chemical_compound ,chemistry ,Electrochemistry ,heterocyclic compounds ,A-DNA ,Adenosine triphosphate ,Spectroscopy ,Hemin - Abstract
Some G-quadruplex-hemin complexes can be used as a type of DNAzymes with peroxidase activity, it can efficiently catalyze H2O2-mediated oxidation reactions. Herein, a simple, colorimetric, and homogenous sensor design strategy based on G-quadruplex-hemin DNAzymes was developed. This strategy takes advantages of the competition between a DNA/DNA duplex and a DNA/target complex, and the formation of catalytically active G-quadruplexes in the presence of targets. With this method, no labeled oligonucleotides are needed, and no expensive instruments are required. This strategy may be developed as a general approach for the analysis of many metal ions and aptamer substrates. In this paper, the feasibility of this strategy in Hg2+ and adenosine triphosphate (ATP) detection was studied, and results showed that this “turn-on” process enabled the selective detection of aqueous Hg2+ and adenosine triphosphate (ATP) using a simple colorimetric technique.
- Published
- 2011