1. Colorimetric detection of class A soybean saponins by coupling DNAzyme with the gap ligase chain reaction
- Author
-
Dingbin Liu, Du Weijun, Yue Aiqin, Jinzhong Zhao, Wenshuai Li, Guorui Wu, and Wang Min
- Subjects
0106 biological sciences ,Detection limit ,0303 health sciences ,Chromatography ,Chemistry ,General Chemical Engineering ,fungi ,Ligase Chain Reaction ,General Engineering ,Deoxyribozyme ,food and beverages ,DNA, Catalytic ,Saponins ,01 natural sciences ,Analytical Chemistry ,Rapid identification ,03 medical and health sciences ,Linear range ,Colorimetry ,Soybeans ,Naked eye ,Ligase chain reaction ,030304 developmental biology ,010606 plant biology & botany - Abstract
Class A saponins are responsible for the taste of soybean products, and the rapid identification of class A saponins from soybean food is essential for both food safety and cultivar screening. In this study, we propose a colorimetric assay based on the coupling of gap ligase chain reaction (Gap-LCR) with DNAzyme to detect the target GmSg-1 genes of class A soybean saponins with the naked eye, without the involvement of expensive instruments. The limits of detection (LODs) for the GmSg-1a and GmSg-1b genes were determined to be 0.1618 and 0.1625 μM, respectively, with a linear range of 0.2-1.2 μM. The DNAzyme-based Gap LCR assay was successfully employed to identify the target genes from different soybean cultivars, providing a simple means for monitoring the quality of soybean products.
- Published
- 2020