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Gemische von Zuckern werden zunachst als Borsaure-Komplexe absteigend in Filtrierpapier einer Elektrophorese unterworfen. Anschliesend werden die einzeln aufgefangenen Fraktionen wie ublich chromatographiert. An zwei Beispielen wird gezeigt, das eine wirkungsvolle Trennung auch mit groseren Durchsatzen erzielt wird. The Separation of Sugar Mixtures by Combined Electrophoresis and Paper Chromatography Mixtures of sugars are subjected as boric acid complexes to electrophoresis in filter paper with a downward flow of the solution. The fractions are collected individually and chromatographed as usual. Two examples show that an effective separation is also achieved in the case of a larger throughput.

2-(1,2-Dihydroxyethyl)-thiamine pyrophosphate (“thiaminepyrophosphate-activated glycolic aldehyde”), isolated from an incubation mixture of hydroxypyruvate and thiamine pyrophosphate with a preparation of pyruvate oxidase from pig-heart muscle, is incubated with purified phosphoketolase ( d -xylulose-5-phosphate d -glyceraldehyde-3-phosphate-lyase (phosphate-acetylating), EC 4.1.2.9), from Lactobacillus plantarum . In the presence of orthophosphate acetyl phosphate is formed, whereas in the presence of ferricyanide the reaction product is glycolic acid. The acetyl phosphate is identified by comparison with authentic acetyl phosphate in high voltage electrophoresis and, after cleavage with hydroxylamine, by comparison with authentic acetylhydroxamic acid in paper chromatography. The reaction rate of “thiamine pyrophosphate-activated glycolic aldehyde” is about 100 times smaller than that of xylulose 5-phosphate, fructose 6-phosphate, or hydroxypyruvate. The possible role of “thiamine pyrophosphate-activated glycolic aldehyde” and its reaction product acetylthiamine pyrophosphate as intermediates in the phosphoketolase-catalyzed reaction is discussed.

Chromatographische Untersuchungen mit Filtrierpapieren mit einem Carboxyl-Gehalt von 1 bis 4% gaben sowohl bei Aminosauren als auch bei Aminen und anorganischen lonen aussichtsreiche Ergebnisse. Chromatography with Carboxyl Paper Chromatographic investigations with filter papers of a carboxyl content of 1 to 4% showed promising results with amino acids as well as amines and inorganic ions.