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Start Over Author "Brown, Tom" Journal angewandte chemie
14 results on '"Brown, Tom"'

5. A Click Chemistry Approach to Targeted DNA Crosslinking with cis‐Platinum(II)‐Modified Triplex‐Forming Oligonucleotides.

Author

Hennessy, Joseph, McGorman, Bríonna, Molphy, Zara, Farrell, Nicholas P., Singleton, Daniel, Brown, Tom, and Kellett, Andrew

Subjects
CISPLATIN, CLICK chemistry, DNA, NUCLEIC acids, BASE pairs, OLIGONUCLEOTIDES, PLATINUM
Abstract

Limitations of clinical platinum(II) therapeutics include systemic toxicity and inherent resistance. Modern approaches, therefore, seek new ways to deliver active platinum(II) to discrete nucleic acid targets. In the field of antigene therapy, triplex‐forming oligonucleotides (TFOs) have attracted interest for their ability to specifically recognise extended duplex DNA targets. Here, we report a click chemistry based approach that combines alkyne‐modified TFOs with azide‐bearing cis‐platinum(II) complexes—based on cisplatin, oxaliplatin, and carboplatin motifs—to generate a library of PtII‐TFO hybrids. These constructs can be assembled modularly and enable directed platinum(II) crosslinking to purine nucleobases on the target sequence under the guidance of the TFO. By covalently incorporating modifications of thiazole orange—a known DNA‐intercalating fluorophore—into PtII‐TFOs constructs, enhanced target binding and discrimination between target and off‐target sequences was achieved. [ABSTRACT FROM AUTHOR]

Published
2022
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6. Electrophilic RNA for Peptidyl-RNA Synthesis and Site-Specific Cross-Linking with tRNA-Binding Enzymes

Author

Fonvielle, Matthieu, primary, Sakkas, Nicolas, additional, Iannazzo, Laura, additional, Le Fournis, Chloé, additional, Patin, Delphine, additional, Mengin-Lecreulx, Dominique, additional, El-Sagheer, Afaf, additional, Braud, Emmanuelle, additional, Cardon, Sébastien, additional, Brown, Tom, additional, Arthur, Michel, additional, and Etheve-Quelquejeu, Mélanie, additional

Published
2016
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10. The Structure of FemXWv in Complex with a Peptidyl‐RNA Conjugate: Mechanism of Aminoacyl Transfer from Ala‐tRNAAla to Peptidoglycan Precursors

Author

Fonvielle, Matthieu, primary, Li de La Sierra‐Gallay, Inés, additional, El‐Sagheer, Afaf H., additional, Lecerf, Maxime, additional, Patin, Delphine, additional, Mellal, Dénia, additional, Mayer, Claudine, additional, Blanot, Didier, additional, Gale, Nittaya, additional, Brown, Tom, additional, van Tilbeurgh, Herman, additional, Ethève‐Quelquejeu, Mélanie, additional, and Arthur, Michel, additional

Published
2013
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14. The Structure of FemXWv in Complex with a Peptidyl-RNA Conjugate: Mechanism of Aminoacyl Transfer from Ala-tRNAAla to Peptidoglycan Precursors.

Author

Fonvielle, Matthieu, Li de La Sierra ‐ Gallay, Inés, El ‐ Sagheer, Afaf H., Lecerf, Maxime, Patin, Delphine, Mellal, Dénia, Mayer, Claudine, Blanot, Didier, Gale, Nittaya, Brown, Tom, van Tilbeurgh, Herman, Ethève ‐ Quelquejeu, Mélanie, and Arthur, Michel

Subjects
PEPTIDYLTRANSFERASE, TRANSFER RNA, PEPTIDOGLYCANS, NUCLEIC acids, AMINOACYLASE
Abstract

Erkenntnisse über den Katalysemechanismus nichtribosomaler Aminosäuretransferasen lieferte die Synthese von Peptidyl ‐ RNA ‐ Konjugaten für die Cokristallisation mit FemXWv aus Weissella viridescens. Die Struktur des Komplexes und Mutationsstudien offenbarten den Mechanismus von Substratbindung, substratvermittelter Katalyse und Stabilisierung der tetraedrisch koordinierten Zwischenstufe in FemXWv. [ABSTRACT FROM AUTHOR]

Published
2013
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