1. Unveiling the Role of DJ-1 Protein in Vesicular Storage and Release of Catecholamine with Nano/Micro-Tip Electrodes
- Author
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Ping Yu, Qingwei Yue, Fei Wu, Wenjie Ma, Meining Zhang, Wenliang Ji, Xianchan Li, Lanqun Mao, Yue Zhang, and Ming Wang
- Subjects
PARK7 Gene ,Protein Deglycase DJ-1 ,Neurotransmission ,010402 general chemistry ,medicine.disease_cause ,01 natural sciences ,Synaptic Transmission ,Catalysis ,Exocytosis ,Protein–protein interaction ,Mice ,Catecholamines ,medicine ,Animals ,Nanotechnology ,Electrodes ,Mice, Knockout ,Mutation ,010405 organic chemistry ,Chemistry ,Vesicle ,General Medicine ,General Chemistry ,0104 chemical sciences ,Cell biology ,Dopamine receptor ,Catecholamine ,medicine.drug - Abstract
DJ-1 protein deficiency caused by PARK7 gene mutation has been suggested to closely relate to Parkinson's disease (PD), mainly through the attenuation D2 dopamine receptor activity in mice; however, whether or how it affects the vesicular storage and exocytosis of neurochemicals remains unclear. By using electrochemical methods at a single vesicle/cell level with nano/micro-tip electrodes, we for the first time find that DJ-1 protein deficiency caused by PARK7 gene knockout (KO) in mice has little effect on vesicular catecholamine content but significantly prolongs the exocytotic events, especially the closing time of exocytotic fusion pores. Further studies suggest the inhibition of α-synuclein aggregation by DJ-1 protein might be one way that DJ-1 protein acts on neurotransmission. This finding offers the first direct link between DJ-1 protein deficiency and vesicular chemical storage and release of chemicals, providing a new chemical insight into the pathology of PD caused by PARK7 gene mutation.
- Published
- 2020