1. The Development of a Fluorescence‐Based Competitive Assay Enabled the Discovery of Dimeric Cyclic Peptide Modulators of Ubiquitin Chains
- Author
-
Ashraf Brik, Roman Meledin, Ganga B. Vamisetti, Mickal Nawatha, and Hiroaki Suga
- Subjects
High-throughput screening ,Peptides | Hot Paper ,Peptide ,010402 general chemistry ,Ligands ,01 natural sciences ,high-throughput screening ,Peptides, Cyclic ,Catalysis ,Fluorescence ,Ubiquitin ,Drug Development ,In vivo ,Protein biosynthesis ,Humans ,chemistry.chemical_classification ,fluorescence-based assay ,biology ,Cell Death ,Dose-Response Relationship, Drug ,010405 organic chemistry ,Communication ,dimeric peptides ,cyclic peptides ,General Medicine ,General Chemistry ,Combinatorial chemistry ,Cyclic peptide ,Communications ,0104 chemical sciences ,High-Throughput Screening Assays ,chemistry ,biology.protein ,peptide engineering ,Conjugate ,HeLa Cells - Abstract
Development of modulators targeting specific interactions of ubiquitin‐based conjugates with their partners is a formidable task since it requires a suitable screening assay and homogeneous ubiquitin conjugates. We developed a novel high‐throughput strategy for screening ligands for Lys48‐linked tetraubiquitin chain in a relatively simple, fast, and affordable manner. This approach combined with a state‐of‐the‐art toolbox of chemical protein synthesis and a specially optimized Cys deprotection protocol enabled us to design highly potent, Lys48‐linked tetraubiquitin chain selective “next generation” dimeric peptide modulators. The dimeric peptide exhibited cancer cell permeability and induced cell death with higher efficiency compared to its monocyclic analogue. These features make our dimeric peptide a promising candidate for further studies using in vivo models. Our assay can be adopted for other various ubiquitin chains in their free or anchored forms as well as conjugates for Ub‐like modifiers., A fluorescence‐based competitive assay was developed, which enabled the high‐throughput screening of various cyclic peptides targeting the Lys48‐linked tetraubiquitin chain. This assay combined with chemical tools enabled the design of a potent dimeric cyclic peptide, which induced cell death with higher efficiency compared to its monocyclic analogue.
- Published
- 2021