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Start Over Author "Peng R" Journal angewandte chemie international edition
15 results on '"Peng R"'

4. Controlled Genetic Encoding of Unnatural Amino Acids in a Protein Nanopore.

Author

Wu, Xue‐Yuan, Li, Meng‐Yin, Yang, Shao‐Jun, Jiang, Jie, Ying, Yi‐Lun, Chen, Peng R., and Long, Yi‐Tao

Subjects
AMINO acids, GENETIC code, PROTEIN engineering, DISCRIMINATION (Sociology), PROTEINS, TRANSFER RNA
Abstract

Conventional protein engineering methods for modifying protein nanopores are typically limited to 20 natural amino acids, which restrict the diversity of the nanopores in structure and function. To enrich the chemical environment inside the nanopore, we employed the genetic code expansion (GCE) technique to site‐specifically incorporate the unnatural amino acid (UAA) into the sensing region of aerolysin nanopores. This approach leveraged the efficient pyrrolysine‐based aminoacyl‐tRNA synthetase‐tRNA pair for a high yield of pore‐forming protein. Both molecular dynamics (MD) simulations and single‐molecule sensing experiments demonstrated that the conformation of UAA residues provided a favorable geometric orientation for the interactions of target molecules and the pore. This rationally designed chemical environment enabled the direct discrimination of multiple peptides containing hydrophobic amino acids. Our work provides a new framework for endowing nanopores with unique sensing properties that are difficult to achieve using classical protein engineering approaches. [ABSTRACT FROM AUTHOR]

Published
2023
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5. Photocatalytic Chemical Crosslinking for Profiling RNA–Protein Interactions in Living Cells

Author

Luo, Huixin, primary, Tang, Wei, additional, Liu, Hongyu, additional, Zeng, Xiangmei, additional, Ngai, William Shu Ching, additional, Gao, Rui, additional, Li, Heyun, additional, Li, Ran, additional, Zheng, Huangtao, additional, Guo, Jianting, additional, Qin, Fangfei, additional, Wang, Gang, additional, Li, Kexin, additional, Fan, Xinyuan, additional, Zou, Peng, additional, and Chen, Peng R., additional

Published
2022
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15. Chemical Remodeling of Cell-Surface Sialic Acids through a Palladium-Triggered Bioorthogonal Elimination Reaction.

Author

Cheng, Bo, Li, Jie, Zhang, Zhaoyue, Hong, Weiyao, Chen, Xing, Chen, Peng R., and Wang, Jie

Subjects
CELL membranes, NEURAMINIC acid, NANOPARTICLES, PALLADIUM catalysts, SIALIC acids, GLYCANS, CARBOXYL group
Abstract

We herein report a chemical decaging strategy for the in situ generation of neuramic acid (Neu), a unique type of sialic acid, on live cells by the use of a palladium-mediated bioorthogonal elimination reaction. Palladium nanoparticles (Pd NPs) were found to be a highly efficient and biocompatible depropargylation catalyst for the direct conversion of metabolically incorporated N-(propargyloxycarbonyl)neuramic acid (Neu5Proc) into Neu on cell-surface glycans. This conversion chemically mimics the enzymatic de-N-acetylation of N-acetylneuramic acid (Neu5Ac), a proposed mechanism for the natural occurrence of Neu on cell-surface glycans. The bioorthogonal elimination was also exploited for the manipulation of cell-surface charge by unmasking the free amine at C5 to neutralize the negatively charged carboxyl group at C1 of sialic acids. [ABSTRACT FROM AUTHOR]

Published
2015
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