1. Backbone Assignment of Fully Protonated Solid Proteins by1H Detection and Ultrafast Magic-Angle-Spinning NMR Spectroscopy
- Author
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Alessandro Marchetti, Lyndon Emsley, Stefan Jehle, Michele Felletti, Gottfried Otting, Anne Lesage, Guido Pintacuda, Michael J. Knight, Ah Young Park, Zhi-Qiang Xu, Nicholas E. Dixon, Yao Wang, ISA - Centre de RMN à très hauts champs, Institut des Sciences Analytiques (ISA), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Scuola Normale Superiore di Pisa (SNS), School of Chemistry [Wollongong], University of Wollongong [Australia], Research School of Chemistry, Australian National University (ANU), Agence Nationale de la Recherche (ANR 08-BLAN-0035-01 and 10-BLAN-713-01), the Joint Research Activity within Research Infrastructures in the 7th Framework program of the EC (BioNMR, contract n. 261863), the Australian Research Council., and European Project
- Subjects
STRUCTURAL BASIS ,magic-angle spinning ,TERMINAL DOMAIN ,Protonation ,DNA polymerase ,010402 general chemistry ,01 natural sciences ,Catalysis ,NMR spectroscopy ,Protein structure ,Magic angle spinning ,Transverse relaxation-optimized spectroscopy ,Spectroscopy ,Nuclear Magnetic Resonance, Biomolecular ,ComputingMilieux_MISCELLANEOUS ,DNA Polymerase III ,Carbon Isotopes ,Nitrogen Isotopes ,010405 organic chemistry ,Chemistry ,scalar transfers ,Proteins ,PEPTIDES ,General Medicine ,General Chemistry ,Nuclear magnetic resonance spectroscopy ,Deuterium ,0104 chemical sciences ,[CHIM.THEO]Chemical Sciences/Theoretical and/or physical chemistry ,Crystallography ,MAS ,RESOLUTION ,STATE NMR ,protein structures ,Proton NMR ,Protons ,Hydrogen - Abstract
Narrow 1H NMR linewidths can be obtained for fully protonated protein samples in the solid state by using ultrafast magic-angle spinning (60 kHz). Medium-size microcrystalline and noncrystalline proteins can be analyzed without any need for deuteration of the protein sample. This approach provides assignments of the backbone 1H, 15N, 13C α, and 13CO resonances and yields information about 1H-1H proximities. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
- Published
- 2012
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