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Enzymatic electrosynthesis has gained more and more interest as an emerging green synthesis platform, particularly for the fixation of CO2. However, the simultaneous utilization of CO2 and a nitrogenous molecule for the enzymatic electrosynthesis of value‐added products has never been reported. In this study, we constructed an in vitro multienzymatic cascade based on the reductive glycine pathway and demonstrated an enzymatic electrocatalytic system that allowed the simultaneous conversion of CO2 and NH3 as the sole carbon and nitrogen sources to synthesize glycine. Through effective coupling and the optimization of electrochemical cofactor regeneration and the multienzymatic cascade reaction, 0.81 mM glycine was yielded with a highest reaction rate of 8.69 mg L−1 h−1 and faradaic efficiency of 96.8 %. These results imply a promising alternative for enzymatic CO2 electroreduction and expand its products to nitrogenous chemicals. [ABSTRACT FROM AUTHOR]

Many existing in vitro biosystems harness power from the chemical energy contained in substrates and co‐substrates, and light or electric energy provided from abiotic parts, leading to a compromise in atom economy, incompatibility between biological and abiotic parts, and most importantly, incapability to spatiotemporally co‐regenerate ATP and NADPH. In this study, we developed a light‐powered in vitro biosystem for poly(3‐hydroxybutyrate) (PHB) synthesis using natural thylakoid membranes (TMs) to regenerate ATP and NADPH for a five‐enzyme cascade. Through effective coupling of cofactor regeneration and mass conversion, 20 mM PHB was yielded from 50 mM sodium acetate with a molar conversion efficiency of carbon of 80.0 % and a light‐energy conversion efficiency of 3.04 %, which are much higher than the efficiencies of similar in vitro PHB synthesis biosystems. This suggests the promise of installing TMs as a green engine to drive more enzyme cascades. [ABSTRACT FROM AUTHOR]

Ammonia Conversion, CO2 Reduction, Enzymatic Electrosynthesis, Multienzymatic Cascades, Reductive Glycine Pathway Keywords: Ammonia Conversion; CO2 Reduction; Enzymatic Electrosynthesis; Multienzymatic Cascades; Reductive Glycine Pathway EN Ammonia Conversion CO2 Reduction Enzymatic Electrosynthesis Multienzymatic Cascades Reductive Glycine Pathway 1 1 1 03/23/23 20230327 NES 230327 B An enzymatic electrocatalytic system b was developed that allows the simultaneous conversion of CO SB 2 sb and NH SB 3 sb for glycine synthesis, as reported by Zhiguang Zhu et al. in their Research Article (e202218387). This system was inspired by the reductive glycine pathway in vivo and is based on an in vitro multienzymatic cascade reaction, representing a novel alternative for enzymatic CO SB 2 sb electroreduction for synthesizing nitrogenous value-added chemicals. [Extracted from the article]

Enzymatic electrosynthesis has gained more and more interest as an emerging green synthesis platform, particularly for the fixation of CO2. However, the simultaneous utilization of CO2 and a nitrogenous molecule for the enzymatic electrosynthesis of value‐added products has never been reported. In this study, we constructed an in vitro multienzymatic cascade based on the reductive glycine pathway and demonstrated an enzymatic electrocatalytic system that allowed the simultaneous conversion of CO2 and NH3 as the sole carbon and nitrogen sources to synthesize glycine. Through effective coupling and the optimization of electrochemical cofactor regeneration and the multienzymatic cascade reaction, 0.81 mM glycine was yielded with a highest reaction rate of 8.69 mg L−1 h−1 and faradaic efficiency of 96.8 %. These results imply a promising alternative for enzymatic CO2 electroreduction and expand its products to nitrogenous chemicals. [ABSTRACT FROM AUTHOR]