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A heterobinuclear Ni-Fe-S centersimilar to that in the hydrogenase from Desulfovibrio gigasis also present in the considerably more complex soluble hydrogenase from the bacterium Alcaligenes eutrophus, as shown by high-resolution X-ray absorption spectroscopy. Reductive activation of this enzyme with NADH leads to a substantial change in the coordination of the nickel, which is probably due to specific substrate properties. A model for the active site after NADH reduction is shown on the right.