24 results on '"Zhao Yuan"'
Search Results
2. Polymorphism in IGFALS gene and its association with scrotal circumference in Hu lambs
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Ma, Zongwu, primary, Wang, Weimin, additional, Zhang, Deyin, additional, Wang, Xinji, additional, Li, Shirong, additional, Zhao, Liming, additional, Zhang, Yukun, additional, Zhao, Yuan, additional, Li, Xiaolong, additional, Lin, Changchun, additional, Wang, Jianghui, additional, Cheng, Jiangbo, additional, Xu, Dan, additional, Yang, Xiaobin, additional, Huang, Yongliang, additional, Cui, Panpan, additional, Liu, Jia, additional, Zeng, Xiwen, additional, Zhai, Rui, additional, Huang, Zhiqiang, additional, Weng, Xiuxiu, additional, and Zhang, Xiaoxue, additional
- Published
- 2024
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3. Polymorphism and expression level of the FADS3 gene and associated with the growth traits in Hu sheep.
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Zeng, Xiwen, Wang, Weimin, Zhang, Deyin, Li, Xiaolong, Zhang, Yukun, Zhao, Yuan, Zhao, Liming, Wang, Jianghui, Xu, Dan, Cheng, Jiangbo, Li, Wenxin, Zhou, Bubo, Lin, Changchun, Yang, Xiaobin, Zhai, Rui, Ma, Zongwu, Liu, Jia, Cui, Panpan, Weng, Xiuxiu, and Wu, Weiwei
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GENE expression ,FATTY acid desaturase ,SHEEP ,UNSATURATED fatty acids ,GENES ,MERINO sheep - Abstract
Growth traits are the economically important traits of sheep, and screening for genes related to growth and development is helpful for the genetic improvement of ovine growth traits. The fatty acid desaturase 3 (FADS3) is one of the important genes affecting the synthesis and accumulation of polyunsaturated fatty acids in animals. In this study, the expression levels of the FADS3 gene and polymorphism of the FADS3 gene associated with growth traits in Hu sheep were detected using quantitative real-time PCR (qRT-PCR), Sanger sequencing, and KAspar assay. The result showed that the expression levels of the FADS3 gene were widely expressed in all tissues, and the expression level of FADS3 in the lung was significantly higher than in other tissues (p <.05). Then, the polymorphism locus g. 2918 A > C was detected in intron 2 of the FADS3 gene, and associated analysis showed that the mutation in the FADS3 gene was associated significantly with growth traits (including body weight, body height, body length, and chest circumference, p <.05). Therefore, individuals with AA genotype showed significantly better growth traits than those with CC genotype, and FADS3 gene could be a candidate gene for improving growth traits in Hu sheep. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
4. Detection of single nucleotide polymorphism in HTR4 and its relationship with growth traits in sheep.
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Xu, Dan, Wang, Xiaojuan, Wang, Weimin, Zhang, Deyin, Li, Xiaolong, Zhang, Yukun, Zhao, Yuan, Cheng, Jiangbo, Zhao, Liming, Wang, Jianghui, Lin, Changchun, Yang, Xiaobin, Weng, Xiuxiu, Zhang, Xiaoxue, and Zheng, Wenxin
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SINGLE nucleotide polymorphisms ,SHEEP breeding ,SHEEP ,LOCUS (Genetics) ,DIGESTIVE organs ,SHEEP breeds ,SMOOTH muscle ,MERINO sheep - Abstract
In this study, a single nucleotide polymorphism of HTR4 (hydroxytryptamine receptor 4) was detected using DNA sequencing and KASPar (Kompetitive Allele-Specific PCR) technique with the aim of analyzing its effect on growth traits in 1102 sheep. A synonymous mutation g.101220 C > T located on the fifth intron of the ovis HTR4 gene was detected, and association analysis showed that this mutation was significantly associated with growth traits in sheep (p <.05), with TT genotypes having significantly lower body weight, height, length and chest circumference than TC and CC genotypes. It showed that the polymorphism of this locus was significantly associated with growth traits in sheep. In addition, qRT-PCR results showed that HTR4 was expressed in different tissues of sheep. It is highly expressed in the liver, spleen and duodenum. As important metabolic, immune and digestive absorption organs in animals, the above tissues can regulate the excitability of intestinal smooth muscle by participating in the body metabolism and nutrient metabolism of sheep, so that sheep can show better growth characteristics. In conclusion, the polymorphic locus identified in HTR4 gene can be used as candidate molecular marker in sheep breeding. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
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5. Polymorphism in ovine ADCY8 gene and its association with residual feed intake in Hu sheep.
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Yang, Xiaobin, Wang, Weimin, Wang, Xiaojuan, Zhang, Deyin, Li, Xiaolong, Zhang, Yukun, Zhao, Yuan, Zhao, Liming, Wang, Jianghui, Xu, Dan, Cheng, Jiangbo, Li, Wenxin, Zhou, Bubo, Lin, Changchun, Zeng, Xiwen, Zhai, Rui, Ma, Zongwu, Liu, Jia, Cui, Panpan, and Zhang, Xiaoxue
- Subjects
SHEEP ,GENE expression ,ADENYLATE cyclase ,GENES ,SINGLE nucleotide polymorphisms ,SHEEP farming ,SHEEP breeds - Abstract
Feed efficiency makes up a large part of sheep production, which also has a crucial impact on the economic benefits of producers. This study explores the polymorphism of Adenylyl cyclase 8 gene associated with feed efficiency and detects the expression characteristics of ADCY8 in ten tissues of Hu sheep. The polymorphism of ADCY8 was recognized by using PCR amplification and Sanger sequencing, KASPar technology was used for genotyping subsequently, and the relationship between SNP and RFI is also studied. The results indicated that an intronic mutation g.24799148 C > T (rs 423395741) was identified in ADCY8, and association analysis showed that the SNP g.24799148 C > T (rs 423395741) was significantly associated with RFI at 100–120, 100–140, 100–160, and 100–180 days (p < 0.05). The quantitative real-time PCR (qRT-PCR) result showed that ADCY8 was expressed in ten tissues, and the expression of ADCY8 gene in rumen tissue was significantly higher than in the other tested tissues. Therefore, these results indicated that the ADCY8 mutation locus may be used as a candidate molecular marker for evaluating the feed efficiency of Hu sheep. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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6. Novel polymorphism at KLF15 gene and its association with growth traits in Hu sheep.
- Author
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Zhai, Rui, Wang, Weimin, Zhang, Deyin, Li, Xiaolong, Zhang, Yukun, Zhao, Yuan, Zhao, Liming, Wang, Jianghui, Xu, Dan, Cheng, Jiangbo, Li, Wenxin, Zhou, Bubo, Lin, Changchun, Zeng, Xiwen, Yang, Xiaobin, Ma, Zongwu, Liu, Jia, Cui, Panpan, and Zhang, Xiao Xue
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SHEEP ,GENETIC polymorphisms ,HEART ,GENE expression ,STATURE ,MYOCARDIUM ,MISSENSE mutation - Abstract
Growth traits are important economic characteristics of livestock and poultry. In the present study, the expression features of KLF15 and the relationship between KLF15 gene polymorphisms and growth traits in Hu sheep were investigated by using real-time quantitative PCR technology (qPCR), Sanger sequencing, and Kaspar genotyping technology. The qPCR results showed that the KLF15 gene is expressed widely in the tested tissues of Hu sheep, and the expression level of the KLF15 gene in the heart and the muscle was significantly higher than in other tissues (p < 0.05). Missense mutation c.62565119 A > G was found in KLF15, and an association analysis showed that it was correlated with the growth traits (body weight, body height, and body length) of Hu sheep (p < 0.05). The body weight, body height, and body length of the sheep carrying the AA genotype were remarkably higher than those of the GG and AG genotypes (p < 0.05). These results showed that novel polymorphisms at the KLF15 gene can be used as a genetic marker of growth traits of Hu sheep. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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7. Estimation of genetic correlations of two key feed efficiency traits with production traits in male Hu sheep.
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Zhao, Yuan, Zhang, Xiaoxue, Li, Fadi, Zhang, Deyin, Zhang, Yukun, Li, Xiaolong, Song, Qizhi, Li, Chong, Zhao, Liming, Wang, Jianghui, Xu, Dan, Cheng, Jiangbo, Li, Wenxin, Lin, Changchun, Zhou, Bubo, and Wang, Weiming
- Subjects
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GENETIC correlations , *SHEEP , *SHEEP breeding , *PRODUCTION losses , *ALIMENTARY canal , *SHEEP farming , *MALES , *RUMEN (Ruminants) - Abstract
Increased body growth and feed efficiency are important for breeding due to expensive feed costs. This study estimated the genetic parameters for two feed efficiency traits [the feed conversion ratio (FCR) and the residual feed intake (RFI)] and other important economic traits in male Hu sheep using 1642 male Hu lambs. The heritability of the RFI was estimated at 0.31 (±0.10)–0.54 (±0.1) and the heritability of the FCR was 0.05 (±0.07)–0.77 (±0.12). There was a negative genetic correlation between rib-eye muscle area traits and RFI. A positive genetic correlation was found between fat deposition traits and feed efficiency traits. The digestive tract weight decreased with the direction of RFI: however, the FCR showed the opposite: A low FCR was accompanied by a high digestive tract weight. The genetic correlation between feed efficiency (RFI and FCR) and rumen weight was negative. Heritability estimates for feed efficiency generate fluctuations with different experimental intervals. The weight of other traits should be reasonably allocated to avoid losses in production and feed efficiency in the selection of feed efficiency traits. The present study added to our understanding of the genetic parameters of sheep under the condition of house feeding. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
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8. Identification of polymorphic loci in OSMR and GHR genes and analysis of their association with growth traits in sheep.
- Author
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Cheng, Jiangbo, Wang, Weimin, Zhang, Deyin, Zhang, Yukun, Li, Xiaolong, Zhao, Yuan, Xu, Dan, Zhao, Liming, Li, Wenxin, Wang, Jianghui, Zhou, Bubo, Lin, Changchun, Yang, Xiaobin, and Zhang, Xiaoxue
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LOCUS (Genetics) ,SHEEP ,SOMATOTROPIN receptors ,GENES ,GENETIC polymorphisms ,SINGLE nucleotide polymorphisms - Abstract
The aim of this study was to analyze the effect of OSMR and GHR genes polymorphisms on growth traits in sheep. The single nucleotide polymorphisms of sheep OSMR and GHR genes were identified by DNA sequencing technology. A total of two intronic mutations g.2443 T > C and g.170196 A > G were identified in OSMR and GHR, respectively. Correlation analysis was carried out between the obtained genotypes and the growth traits of sheep. The results showed that at the OSMR g.2443 T > C locus, the body weight, chest circumference and cannon circumference of the TT genotype sheep were significantly higher than those of the CC genotype sheep (p <.05). At the GHR g.170196 A > G locus, the body weight, body length, chest circumference and cannon circumference of the AA genotype sheep were significantly higher than those of the AG genotype and GG genotype sheep (p <.05). Moreover, the body weight of sheep of combination TT
OSMR /AAGHR genotype was significantly higher than that of other combination genotypes (p <.05). Therefore, we believe that the polymorphic sites identified in the OSMR and GHR genes can be used as candidate molecular markers for breeding good traits in sheep. [ABSTRACT FROM AUTHOR]- Published
- 2023
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9. Polymorphism of sheep PRKAA2 gene and its association with growth traits.
- Author
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Li, Wenxin, Wang, Xiaojuan, Zhang, Xiaoxue, Li, Fadi, Zhang, Deyin, Li, Xiaolong, Zhang, Yukun, Zhao, Yuan, Zhao, Liming, Xu, Dan, Cheng, Jiangbo, Wang, Jianghui, Zhou, Bubo, Lin, Changchun, and Wang, Weimin
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PESTE des petits ruminants ,SINGLE nucleotide polymorphisms ,SHEEP ,GENE expression ,BODY weight ,GENES - Abstract
Small ruminants farming plays an important role in the livelihood of a large part of the population. Herein we aimed to analyze the effects of single nucleotide polymorphisms in PRKAA2 gene on the growth-related traits of Hu sheep and Dorper sheep. The body weight and body type of 1254 sheep were measured at 80, 100, 120, 140, 160 and 180d, and 37620 phenotypic data were collected. RT-qPCR analysis was performed to test PRKAA2 gene expressed in different tissues of sheep, with the highest expression level in spleen, followed by kidney. In the present study, the PRKAA2 gene sequencing revealed one polymorphism located on Chr1 (Oar_rambouillet_v1.0), termed as chr1:32832382 G > A, and were significantly associated with growth traits of sheep (p < 0.05). The body weight, body length, chest circumference, and cannon circumference of individuals with AA genotype were significantly higher than those with the GG and GA genotypes (p < 0.05). Our findings reveal that PRKAA2 gene could be used as a marker-assisted selection to improve the growth-related traits of sheep. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
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10. Expression and polymorphisms of CD8B gene and its associations with body weight and size traits in sheep.
- Author
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Lin, Changchun, Li, Fadi, Zhang, Xiaoxue, Zhang, Deyin, Li, Xiaolong, Zhang, Yukun, Zhao, Yuan, Song, Qizhi, Wang, Jianghui, Zhou, Bubo, Cheng, Jiangbo, Xu, Dan, Li, Wenxin, Zhao, Liming, and Wang, Weimin
- Subjects
GENE expression ,BODY size ,BODY weight ,GENETIC polymorphisms ,SINGLE nucleotide polymorphisms ,SHEEP breeding ,SHEEP breeds - Abstract
The growth traits are economically important traits in sheep. Improving growth rates will increase the profitability of producers. The aim of this study was to identify alleles of CD8B (encoding T-cell surface glycoprotein CD8 beta chain) that are aberrantly expressed in different tissues and to assess the effects and associations of its different genotypes on weight and size traits in sheep. Using quantitative real-time reverse transcription PCR arrays, expression profiling of CD8B was performed in various organs and tissues. CD8B was ubiquitously expressed, with very high expression in the lung, spleen, lymph, duodenum, and liver. One intronic mutation (chr3:62,718,030 (Oar_rambouillet_v1.0, same below) G > A) was identified using pooled DNA sequencing. Subsequently, the variants (AA, AG, and GG) were genotyped using the KASPar
® PCR single nucleotide polymorphism (SNP) genotyping system. The results of association analysis with body weight and body size traits in 1304 sheep showed that increases in multiple phenotypic traits correlated with the AA genotype (body weight, p < 0.05; body length, p < 0.05). Thus, SNP chr3:62,718,030 G > A is a promising molecular marker for marker-assisted selection in sheep breeding. [ABSTRACT FROM AUTHOR]- Published
- 2023
- Full Text
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11. Polymorphisms in the ovine GP5 gene associated with blood physiological indices.
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Xu, Dan, Wang, Xiaojuan, Zhang, Xiaoxue, Li, Fadi, Zhang, Deyin, Li, Xiaolong, Zhang, Yukun, Zhao, Yuan, Song, Qizhi, Cheng, Jiangbo, Zhao, Liming, Wang, Jianghui, Li, Wenxin, Zhou, Bubo, Lin, Changchun, and Wang, Weimin
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BLOOD cell count ,LEUCOCYTES ,MEAN platelet volume ,SINGLE nucleotide polymorphisms ,ERYTHROCYTES ,PLATELET count ,NEUTROPHILS - Abstract
This study aimed to analyze the effects of polymorphisms in GP5 on blood physiological indices of 1065 sheep. The coefficients of variation of the red blood cell count (RBC), hemoglobin concentration (HGB), mean platelet volume (MPV), mean erythrocyte hemoglobin content (MCH), and red blood cell distribution-coefficient of variation (RDW-CV) were greater than 10%, and there was a very significant correlation between the main indices such as RBC, white blood cell, and platelet count (PLT) and most other indices (p < 0.01). qRT-PCR showed that GP5 was expressed in the heart, liver, spleen, lung, kidney, rumen, duodenum, muscle, tail fat, and lymph tissue, with significantly higher expression in the lymph. Subsequently, we detected single nucleotide polymorphisms (SNPs) in GP5 from group, which identified synonymous mutation g.657 T > C in the first exon of GP5. Association analysis showed significant correlations between the SNP and the physiological traits (p < 0.05), in which the RBC, neutrophilic granulocyte (NEUT) and RDW-CV values in sheep with the TC genotype and TT genotype were markedly lower than those in the CC genotype (p < 0.05). Thus, GP5 polymorphisms could be candidate biomarkers to screen blood physiological indices. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
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12. Polymorphism and expression of the HMGA1 gene and association with tail fat deposition in Hu sheep.
- Author
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Wang, Jianghui, Zhang, Xiaoxue, Wang, Xiaojuan, Li, Fadi, Zhang, Deyin, Li, Xiaolong, Zhang, Yukun, Zhao, Yuan, Song, Qizhi, Zhao, Liming, Xu, Dan, Cheng, Jiangbo, Li, Wenxin, Zhou, Bubo, Lin, Changchun, and Wang, Weimin
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SINGLE nucleotide polymorphisms ,SHEEP ,HEART ,GENE expression ,FAT ,ORGANS (Anatomy) ,GENETIC markers - Abstract
Hu sheep is an excellent short fat-tailed breed in China. Fat deposition in Hu sheep tail affects carcass quality and consumes a lot of energy, leading to an increase in feed cost. The objective of this study was to analyze the effects of HMGA1 polymorphism on tail fat weight in Hu sheep. Partial coding and non-coding sequences of HMGA1 were amplified with PCR and single nucleotide polymorphisms (SNP) of HMGA1 in 1163 Hu sheep were detected using DNA sequencing and KASPar technology. RT-qPCR analysis was performed to test HMGA1 expression in different tissues. The results showed that the expression of HMGA1 was higher in the duodenum, liver, spleen, kidney, and lung than in the heart, muscle, rumen, tail fat, and lymph. A mutation, g.5312 C > T, was detected in HMGA1; g.5312 C > T was significantly associated with tail fat weight, relative weight of tail fat (body weight), and relative weight of tail fat (carcass) (p < 0.05). The tail fat weight of the TT genotype was remarkably higher than that of the CC and TC genotypes. Therefore, HMGA1 can be used as a genetic marker for marker-assisted selection of tail fat weight in Hu sheep. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
13. Polymorphism in ovine ADCY8 gene and its association with residual feed intake in Hu sheep
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Yang, Xiaobin, primary, Wang, Weimin, additional, Wang, Xiaojuan, additional, Zhang, Deyin, additional, Li, Xiaolong, additional, Zhang, Yukun, additional, Zhao, Yuan, additional, Zhao, Liming, additional, Wang, Jianghui, additional, Xu, Dan, additional, Cheng, Jiangbo, additional, Li, Wenxin, additional, Zhou, Bubo, additional, Lin, Changchun, additional, Zeng, Xiwen, additional, Zhai, Rui, additional, Ma, Zongwu, additional, Liu, Jia, additional, Cui, Panpan, additional, and Zhang, Xiaoxue, additional
- Published
- 2022
- Full Text
- View/download PDF
14. Novel polymorphism at KLF15 gene and its association with growth traits in Hu sheep
- Author
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Zhai, Rui, primary, Wang, Weimin, additional, Zhang, Deyin, additional, Li, Xiaolong, additional, Zhang, Yukun, additional, Zhao, Yuan, additional, Zhao, Liming, additional, Wang, Jianghui, additional, Xu, Dan, additional, Cheng, Jiangbo, additional, Li, Wenxin, additional, Zhou, Bubo, additional, Lin, Changchun, additional, Zeng, Xiwen, additional, Yang, Xiaobin, additional, Ma, Zongwu, additional, Liu, Jia, additional, Cui, Panpan, additional, and Zhang, Xiao Xue, additional
- Published
- 2022
- Full Text
- View/download PDF
15. Identification of polymorphic loci in OSMR and GHR genes and analysis of their association with growth traits in sheep
- Author
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Cheng, Jiangbo, primary, Wang, Weimin, additional, Zhang, Deyin, additional, Zhang, Yukun, additional, Li, Xiaolong, additional, Zhao, Yuan, additional, Xu, Dan, additional, Zhao, Liming, additional, Li, Wenxin, additional, Wang, Jianghui, additional, Zhou, Bubo, additional, Lin, Changchun, additional, Yang, Xiaobin, additional, and Zhang, Xiaoxue, additional
- Published
- 2022
- Full Text
- View/download PDF
16. Effect of feed efficiency on growth performance, body composition, and fat deposition in growing Hu lambs.
- Author
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Zhang, Xiaoxue, Li, Guoze, Li, Fadi, Zhang, Deyin, Yuan, Lvfeng, Zhao, Yuan, Zhang, Yukun, Li, Xiaolong, Song, Qizhi, and Wang, Weimin
- Subjects
BODY composition ,LAMBS ,FAT ,ANIMAL feeds ,BODY weight ,SHEEP ,JEJUNUM - Abstract
The present study aimed to investigate the relationship between growth performance, body composition, and fat deposition factors, and feed efficiency in growing lambs. We measured average daily feed intake (ADFI) and body weight (BW) from 653 Hu sheep that were fed a pellet diet. The residual feed intake (RFI) not significantly genetic and phenotypic correlated with the metabolic body weight (MBW) and average daily gain (ADG), but it was significantly genetic and phenotypic correlated with ADFI and the feed conversion ratio (FCR) (p < 0.01). However, the FCR was significantly associated with growth traits (p < 0.01). With the same ADG, body fat deposition was greater in animals with low feed efficiency compared with high feed efficiency. Therefore, excessive fat deposition can affect the feed efficiency of the body, and organ weight and gut-weight have a greater impact on the feed efficiency of lambs. The reticulum stomach and jejunum of lambs with a low RFI were smaller compared with that in the high RFI, indicating that lambs with a low RFI have less intake and a higher absorption rate. Small organs, such as the liver, of lambs with high FE might be associated with low energy expenditure and slow metabolism. This study provides a new perspective to study the biological processes responsible for feed efficiency variation in lambs. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
17. Polymorphism of sheep PRKAA2 gene and its association with growth traits
- Author
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Li, Wenxin, primary, Wang, Xiaojuan, additional, Zhang, Xiaoxue, additional, Li, Fadi, additional, Zhang, Deyin, additional, Li, Xiaolong, additional, Zhang, Yukun, additional, Zhao, Yuan, additional, Zhao, Liming, additional, Xu, Dan, additional, Cheng, Jiangbo, additional, Wang, Jianghui, additional, Zhou, Bubo, additional, Lin, Changchun, additional, and Wang, Weimin, additional
- Published
- 2021
- Full Text
- View/download PDF
18. Expression and polymorphisms of CD8B gene and its associations with body weight and size traits in sheep
- Author
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Lin, Changchun, primary, Li, Fadi, additional, Zhang, Xiaoxue, additional, Zhang, Deyin, additional, Li, Xiaolong, additional, Zhang, Yukun, additional, Zhao, Yuan, additional, Song, Qizhi, additional, Wang, Jianghui, additional, Zhou, Bubo, additional, Cheng, Jiangbo, additional, Xu, Dan, additional, Li, Wenxin, additional, Zhao, Liming, additional, and Wang, Weimin, additional
- Published
- 2021
- Full Text
- View/download PDF
19. Polymorphisms in the ovine GP5 gene associated with blood physiological indices
- Author
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Xu, Dan, primary, Wang, Xiaojuan, additional, Zhang, Xiaoxue, additional, Li, Fadi, additional, Zhang, Deyin, additional, Li, Xiaolong, additional, Zhang, Yukun, additional, Zhao, Yuan, additional, Song, Qizhi, additional, Cheng, Jiangbo, additional, Zhao, Liming, additional, Wang, Jianghui, additional, Li, Wenxin, additional, Zhou, Bubo, additional, Lin, Changchun, additional, and Wang, Weimin, additional
- Published
- 2021
- Full Text
- View/download PDF
20. Polymorphism and expression of the HMGA1 gene and association with tail fat deposition in Hu sheep
- Author
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Wang, Jianghui, primary, Zhang, Xiaoxue, additional, Wang, Xiaojuan, additional, Li, Fadi, additional, Zhang, Deyin, additional, Li, Xiaolong, additional, Zhang, Yukun, additional, Zhao, Yuan, additional, Song, Qizhi, additional, Zhao, Liming, additional, Xu, Dan, additional, Cheng, Jiangbo, additional, Li, Wenxin, additional, Zhou, Bubo, additional, Lin, Changchun, additional, and Wang, Weimin, additional
- Published
- 2021
- Full Text
- View/download PDF
21. Effect of feed efficiency on growth performance, body composition, and fat deposition in growing Hu lambs
- Author
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Zhang, Xiaoxue, primary, Li, Guoze, additional, Li, Fadi, additional, Zhang, Deyin, additional, Yuan, Lvfeng, additional, Zhao, Yuan, additional, Zhang, Yukun, additional, Li, Xiaolong, additional, Song, Qizhi, additional, and Wang, Weimin, additional
- Published
- 2021
- Full Text
- View/download PDF
22. The association of polymorphisms in the ovine PPARGC1B and ZEB2 genes with body weight in Hu sheep.
- Author
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Zhang, Deyin, Zhang, Xiaoxue, Li, Fadi, La, Yongfu, Li, Guoze, Zhang, Yukun, Li, Xiaolong, Zhao, Yuan, Song, Qizhi, and Wang, Weimin
- Subjects
BODY weight ,SINGLE nucleotide polymorphisms ,SHEEP ,SHEEP breeds ,GENES ,DNA sequencing - Abstract
The aims of this study were to analyze the effects of PPARGC1B and ZEB2 polymorphisms on the body weight of Hu sheep. DNA sequencing and KASPar technologies were used to detect single nucleotide polymorphisms (SNPs) within the PPARGC1B and ZEB2 genes of Hu sheep (n = 207). Two synonymous mutations, g.300 G > A and g.645 C > T, were detected in PPARGC1B and ZEB2, respectively. The body weights of sheep were recorded at 80, 100, 120, 140, 160 and 180-days, and associations between these polymorphisms and body weight changes were analyzed. Association analysis demonstrated that the polymorphisms in PPARGC1B and ZEB2 significantly associated with body weight (p < 0.05). At the g.300 G > A locus, individuals with the GG genotype had significantly higher body weight than those with the AA genotype, and at the g.645 C > T locus, individuals with the TT genotype had significantly higher body weight than those with the TC genotype. Individuals with both polymorphisms exhibited significantly different growth (p < 0.05). These data suggest that polymorphisms in the PPARGC1B and ZEB2 genes can be used as candidate molecular markers for the breeding of desirable growth traits in Hu sheep. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
23. The association of polymorphisms in the ovine PPARGC1B and ZEB2 genes with body weight in Hu sheep
- Author
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Zhang, Deyin, primary, Zhang, Xiaoxue, additional, Li, Fadi, additional, La, Yongfu, additional, Li, Guoze, additional, Zhang, Yukun, additional, Li, Xiaolong, additional, Zhao, Yuan, additional, Song, Qizhi, additional, and Wang, Weimin, additional
- Published
- 2020
- Full Text
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24. Polymorphism in <italic>IGFALS</italic> gene and its association with scrotal circumference in Hu lambs.
- Author
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Ma, Zongwu, Wang, Weimin, Zhang, Deyin, Wang, Xinji, Li, Shirong, Zhao, Liming, Zhang, Yukun, Zhao, Yuan, Li, Xiaolong, Lin, Changchun, Wang, Jianghui, Cheng, Jiangbo, Xu, Dan, Yang, Xiaobin, Huang, Yongliang, Cui, Panpan, Liu, Jia, Zeng, Xiwen, Zhai, Rui, and Huang, Zhiqiang
- Abstract
AbstractScrotal circumference is an important reproductive index of breeding rams, which has a high genetic correlation with ejaculation volume and semen quality. In this study, the scrotal circumference of 1353 male Hu sheep at different stages of development was measured and descriptive statistical analysis was performed. The results showed that the coefficient of variation of scrotal circumference at each stage was greater than 10%, and its heritability were moderately to high, ranging from 0.318 to 0.719. We used PCR amplification and Sanger sequencing to scan the polymorphisms of the
IGFALS gene, and performed association analysis with the circumference of the scrotum at different stages. We identified a synonymous mutation g.918 G > C in exon 1 of theIGFALS gene, and this mutation was significantly associated with scrotal circumference at 100, 120, 140, 160 and 180 days (p < 0.05). Therefore,IGFALS gene polymorphism can be used as a molecular marker affecting scrotal circumference of Hu sheep, which can provide a reference for future molecular marker-assisted selection of scrotal circumference in sheep. [ABSTRACT FROM AUTHOR]- Published
- 2024
- Full Text
- View/download PDF
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