1. Precise mapping of breakpoints in conserved synteny between human chromosome 1 and pig chromosomes 4, 6 and 9
- Author
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Patrick Chardon, H. S. Sun, Martine Yerle, Philippe Pinton, A. Goureau, Carolyn Fitzsimmons, and Christopher K. Tuggle
- Subjects
Genetics ,Yeast artificial chromosome ,education.field_of_study ,POU domain ,Chromosome ,General Medicine ,Quantitative trait locus ,Biology ,Homology (biology) ,Ribosomal protein L5 ,Sequence-tagged site ,Animal Science and Zoology ,education ,Gene - Abstract
Previous comparative mapping suggested that at least five pig chromosomes (Sscr4, 6, 9, 10 and 14) share homology with human chromosome 1 (Hsapl). A significant quantitative trait loci (QTL) for fat deposition has been identified on Sscr4 that appears to be near the junction region between Sscr4 and Sscr9 relative to Hsapl. It is of interest to define the boundaries of conserved synteny between pig chromosomes and Hsapl to use human map information to identify putative comparative positional candidates for this QTL. Eleven genes, including Janus kinase 1 (JAK1), Prostaglandin E receptor3 (PTGER3), urate oxidase (UOX), coagulation factor 3 (F3), vascular cell adhesion molecule 1 (VCAM1), ribosomal protein L5 (RPL5), POU domain, class 2, transcription factor 1 (POU2F1), coagulation factor 5 (F5), Prostaglandin endoper-oxide synthase-2 (PTGS2), myosin binding protein H (MYBPH) and Antithrombin III (SERPINC1), were selected to refine the boundaries of the blocks of conserved synteny between Hsap1 and pig chromosomes. Pig sequence tagged sites (STSs) were developed and used to physically map these 11 genes using a somatic cell hybrid panel. Eight loci have been mapped by using fluorescent in situ hybridization (FISH) to improve map resolution. Heterologous FISH was used to refine the location of VCAM1 on human chromosomes. In addition, human yeast artificial chromosomes (YACs) were mapped by heterologous FISH on pig metaphases to refine the boundaries of the regions of homology between Sscr4 and Sscr9 on Hsapl. Results from this study suggest the precise break in conserved synteny on Hsapl corresponding to the Sscr4/6 and Sscr4/9 transitions are most likely on the Hsap1p22 and Hsaplq24-25 regions, respectively. Further, our data predict that Hsaplq21-24 is a candidate region for the backfat QTL localized to Sscr4.
- Published
- 2002