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Restricted nutritional consumption during the peri-conceptional period affects the potential for DNA methylation and alters endometrial transcriptomic profile during the peri-implantation period. The restricted diet fed to females during the peri-conceptional period may affect the transcriptomic profile in peri-implantation embryos. In the present study, the transcriptome of embryos of normal-diet-fed gilts was determined and compared with that in embryos of restricted-diet-fed gilts during the peri-implantation period. The restricted-diet-fed gilts were fed forage, in which the dose of proteins and energy had been reduced by 30% compared to the normal diet (Polish Norms of Nutrition). To clarify the issue Agilent's Porcine (V2) Two-Color Gene Expression Microarray 4 × 44 was used. Analysis of the microarray data revealed that the expression of 787 genes with known biological function were consistently altered (496 up- and 291 down-regulated) in embryos. The accurately annotated genes were organized into five categories and 18 subcategories containing 62 biological pathways. The qPCR analysis of ten selected genes [i.e., 5 acid phosphatase, tartrate resistant (ACP5), high mobility group box 2 (HMGB2), prostaglandin-endoperoxide synthase 2 (PTGS2), arachidonate 12-lipoxygenase (ALOX12), adiponectin receptor 2 (ADIPOR2), DNA (cytosine-5)-methyltransferase 1 (DNMT1), steroidogenic acute regulatory protein (STAR), progesterone receptor membrane component 2 (PGRMC2), progestin and adipoQ receptor family member 7 (PAQR7) and serpin family A member 1 (SERPINA1)] confirmed altered gene expression in embryos of restricted-diet-fed gilts. The insight into embryonic transcriptome indicates that female under-nutrition during the peri-conceptional period may create alterations in the pattern of genes expressed in the peri-implantation embryos., (Copyright © 2018. Published by Elsevier B.V.)

The optimal environment in the oviduct is created by adjusting its ultrastructure and secretory capacity to protect gametes and embryos. It was hypothesized that direct contact between the isthmic epithelium and 2- and 4-cell-stage embryos would alter the transcriptomic profile of the isthmus in pigs. Microarray analysis was performed to determine the alterations in gene expression of the isthmus on Days 2-3 of pregnancy in pigs (after natural mating) during embryo presence in the oviduct. Of 43,803 microarray probes, 354 (0.81%) transcripts were altered (P-value ≤ 0.05 and fold-change ≥ 1.2) on the days of pregnancy when assessments were made. Of these 354 transcripts, 118 (33.3%) were up-regulated, and 236 (66.7%) were down-regulated. A total of 57 (48.3%) up-regulated and 73 down-regulated (30.9%) transcripts were classified into gene ontology categories. Of the 354 altered genes, 36 (10.2%) were categorized into the Toll-like or NOD-like receptor signaling pathway, in the immune system subcategory. Selected genes engaged in maternal immune function were down-regulated. The up-regulated genes were involved in epigenetic regulation, the protection of embryos against oxidative stress and xenobiotics and the control of estrogen metabolism. The 2- and 4-cell-stage embryos might, therefore, affect the oviductal transcriptome to optimize the intra-oviductal milieu, which is necessary to support proper development of embryos. The results of this study indicates the pig oviduct has the capacity to alter its transcriptomic profile as a result of early embryo development after natural mating., (Copyright © 2018 Elsevier B.V. All rights reserved.)