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1. Proteomic Analysis of Beef Tenderloin and Flank Assessed Using an Isobaric Tag for Relative and Absolute Quantitation (iTRAQ)

Author

Zhaomin Lei, Jianping Wu, Deyin Zhang, Ting Liu, Shengguo Zhao, Jianfu Wang, and Xiaoxue Zhang

Subjects
itraq, beef, proteomics, differential expression, go analysis, kegg pathway analysis, tenderloin steak, flank steak, Veterinary medicine, SF600-1100, Zoology, QL1-991
Abstract

Herein, we performed a proteomic analysis of tenderloin and flank steaks from Simmental cattle using the isobaric tags for a relative and absolute quantification (iTRAQ) approach. We identified 17 amino acids in both steaks, and Gly, Cys, Ile, Lys, and Pro differed most in abundance between the steak types (p < 0.05). A comparison of the expression patterns in steaks revealed 128 differentially expressed proteins (DEPs), of which 44 were up-regulated and 84 were down-regulated. Furthermore, 27 DEPs (p < 0.05) were subjected to gene ontology (GO) analysis, and many were found to be related to oxidation-reduction, metabolism, hydrogen ion transmembrane transport, transport, the tricarboxylic acid (TCA) cycle, mitochondrial electron transport, and the conversion of nicotinamide adenine dinucleotide (NADH) to ubiquinone. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis also implicated these DEPs in various signalling pathways, including oxidative phosphorylation, cardiac muscle contraction, the TCA cycle, biosynthesis, and the metabolism. These findings provide a new insight into key proteins involved in the determination of amino acid composition in beef.

Published
2020
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2. Proteomic Analysis of Beef Tenderloin and Flank Assessed Using an Isobaric Tag for Relative and Absolute Quantitation (iTRAQ)

Author

Deyin Zhang, Shengguo Zhao, Xiaoxue Zhang, Ting Liu, Zhaomin Lei, Jianping Wu, and Jianfu Wang

Subjects
itraq, kegg pathway analysis, Oxidative phosphorylation, Nicotinamide adenine dinucleotide, tenderloin steak, Article, differential expression, chemistry.chemical_compound, proteomics, lcsh:Zoology, lcsh:QL1-991, KEGG, chemistry.chemical_classification, lcsh:Veterinary medicine, General Veterinary, Metabolism, beef, Hydrogen ion transmembrane transport, Amino acid, Citric acid cycle, chemistry, Biochemistry, lcsh:SF600-1100, Animal Science and Zoology, go analysis, flank steak, Isobaric tag for relative and absolute quantitation
Abstract

Herein, we performed a proteomic analysis of tenderloin and flank steaks from Simmental cattle using the isobaric tags for a relative and absolute quantification (iTRAQ) approach. We identified 17 amino acids in both steaks, and Gly, Cys, Ile, Lys, and Pro differed most in abundance between the steak types (p &lt, 0.05). A comparison of the expression patterns in steaks revealed 128 differentially expressed proteins (DEPs), of which 44 were up-regulated and 84 were down-regulated. Furthermore, 27 DEPs (p &lt, 0.05) were subjected to gene ontology (GO) analysis, and many were found to be related to oxidation-reduction, metabolism, hydrogen ion transmembrane transport, transport, the tricarboxylic acid (TCA) cycle, mitochondrial electron transport, and the conversion of nicotinamide adenine dinucleotide (NADH) to ubiquinone. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis also implicated these DEPs in various signalling pathways, including oxidative phosphorylation, cardiac muscle contraction, the TCA cycle, biosynthesis, and the metabolism. These findings provide a new insight into key proteins involved in the determination of amino acid composition in beef.

Published
2020

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