1. TPA stimulation culture for improved detection of t(11;14)(q13;q32) in mantle cell lymphoma
- Author
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Jean-Hugues Patte, Jean-Luc Laï, Emmanuelle Barouk-Simonet, Claude Preudhomme, Marie-Christine Copin, Bruno Quesnel, Joris Andrieux, Pauline Huyghe, and Nathalie Grardel-Duflos
- Subjects
Adult ,Male ,medicine.medical_specialty ,Cell Culture Techniques ,Chromosomal translocation ,Stimulation ,Lymphoma, Mantle-Cell ,Biology ,Translocation, Genetic ,immune system diseases ,hemic and lymphatic diseases ,Genetics ,medicine ,Tumor Cells, Cultured ,Humans ,neoplasms ,Aged ,Chromosomes, Human, Pair 14 ,Chromosomes, Human, Pair 11 ,Cytogenetics ,Middle Aged ,medicine.disease ,Molecular biology ,medicine.anatomical_structure ,Cell culture ,Tetradecanoylphorbol Acetate ,Mantle cell lymphoma ,Female ,Bone marrow - Abstract
Cytogenetic analysis of mantle cell lymphoma (MCL), characterized by the presence of t(11;14)(q13;q32) translocation, is often difficult because of the low proliferating rate of MCL cells and the presence of normal cells in bone marrow which may interfere with growth of MCL cells. We describe herein a TPA (12-O-tetradecanoylphorbol 13-acetate) stimulated culture to improve detection of t(11;14)(q13;q32) in 20 MCL patients regardless of the samples used.
- Published
- 2002