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The investigations were carried out on healthy young males aged 20-21 years. Those young men, whose previously different diets were determined by the dietary habits of their families, were selected and grouped under uniform conditions (feeding, housing, physical activity). After 4 months staying under these uniform conditions their diet was changed adding E.F.A. to their previous food rations. Food rations enriched with E.F.A. were given to these young males for 3 months. Then E.F.A. additions to the diet was stopped and the observed subjects received food rations, identical as during the initial 4-month period prior to E.F.A. enrichment, these rations were given during 5 months. Immediately after grouping these men were subjected to biochemical investigation of the serum and to anthropometric and general medical examinations (examination I). The subsequent examinations (II, III, IV) were carried out while the subjects were living under uniform conditions: II) after 4 months on the diet without E.F.A. enrichment, III) after 3 months on the diet enriched with E.F.A., and IV) after 5 months of diet without E.F.A. enrichment (after withdrawal of additional E.F.A.). The investigations were begun on 97 young males (examination I). The consecutive examinations (II, III, IV) were carried out on smaller groups of subjects for reasons on with the authors had no influence. During the experiment, 2-3 times in a month, sample of daily food rations given to the studied subjects and residual food on the plates were taken for analysis. This was done for determination of actual food intake. Through the whole period of the experiment the observed subjects were inquired about additional food consumed. The biochemical investigations of the nutritional state of these young subjects included hematological indices, serum proteins, selected biochemical components in the serum, activity of certain enzymes in the serum, serum lipid components and serum level of certain vitamins. The results of these investigations show the enrichment of diets during 3 months with vegetable fats (sunflower oil, margarine containing 40% of E.F.A.) for increasing the amount of E.F.A. in the whole daily food (together with additional food calculated on the basis of inquiry information) from 14.7 g to 27.7 g (3,2% kcal and 5,8% kcal respectively) has a favorable effect on selected serum lipids. The following changes appeared: decrease of total lipid level, total cholesterol level, cholesterol content of beta and prebeta-lipoproteins levels, free fatty acid level and the favorable changes in the composition of fatty acid in total lipid and selected lipid fractions in the serum. Food enrichment with E.F.A. led to rise the level of linoleic acid and total E.F.A. level and to fall the level of saturated fatty acids. The ratio of polyunsaturated to saturated fatty acids changes favorably both in total serum lipids and in different lipid fraction...