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To investigate gravity wave (GW) perturbations in the midlatitude mesopause region during boreal equinox, 433 h of continuous Na lidar full diurnal cycle temperature measurements in September between 2011 and 2015 are utilized to derive the monthly profiles of GW-induced temperature variance, T′², and the potential energy density (PED). Operating at Utah State University (42° N, 112° W), these lidar measurements reveal severe GW dissipation near 90 km, where both parameters drop to their minima (~20 K² and ~50m² s-2, respectively). The study also shows that GWs with periods of 3-5 h dominate the midlatitude mesopause region during the summer-winter transition. To derive the precise temperature perturbations a new tide removal algorithm suitable for all ground-based observations is developed to de-trend the lidar temperature measurements and to isolate GW-induced perturbations. It removes the tidal perturbations completely and provides the most accurate GW perturbations for the ground-based observations. This algorithm is validated by comparing the true GW perturbations in the latest mesoscale-resolving Whole Atmosphere Community Climate Model (WACCM) with those derived from the WACCM local outputs by applying this newly developed tidal removal algorithm. [ABSTRACT FROM AUTHOR]