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The purpose of the study was to determine the histopathological and In-vivo study of Moringa Oleifera Seed of moringa oleifera seed dietary. Fifty (50) albino rats were obtained from faculty of Health Science animal breeding centre, Obafemi Awolowo University, Ile-Ife, Nigeria. They were weighed and grouped into five groups of ten each and fed with five dietary samples for 28 days. Dietary samples investigated consisted of basal dietary, a nitrogen free diet 100% (1) control dietary (2) basal 80%: pressure cooked moringa seed 10%: soybean 10% (3) basal 80%: oven roasted moringa seed 10: soybean 10% (4) basal 80%: raw moringa seed 10%: Soybean 10% (5). The result revealed that histopathological study including spleen, heart, lungs, kidney, liver were healthy and chemical analysis such as pH, titratable acidity%, brix% and vitamin Cmg/100g were comparable with control samples, They were ranged from 6.05-6.60, 0.01-0.04%, 5-10% and 22.5-28.5 Cmg/100g. Proximate composition includes protein%, moisture%, fat%, ash%, crude fiber%, CHO% and caloric values. They were ranged from 10.20-10.30%, 4.25-4.34%, 2.68-3.80%, 2.30-2.64%, 0.68-0.88%, 79.07-88.85%, 382-389 Kcal% respectively. Histopathological and In-vivo study of formulated moringa samples dietary1-5 showed to improve health and promote growth. The tissues showed no infarcted zone, no oedema, no inflammatory cells and separation of cardiac muscle fibres, no cellular disintegration, and no toxicity symptom hence fit for human consumption. [ABSTRACT FROM AUTHOR]