1. Human developmental anatomy: Microscopic magnetic resonance imaging (μMRI) of four human embryos (from Carnegie Stage 10 to 20)
- Author
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Jean-Marc Nuzillard, C. Avisse, Marc Braun, Romain Tonnelet, Yohann Renard, Hervé Kaplan, Martin Lhuaire, Marc Labrousse, Agathe Martinez, Faculté de Médecine de Reims, Institut de Chimie Moléculaire de Reims - UMR 7312 (ICMR), SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Service d’Anatomie Pathologique [CHRU Nancy], Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy), Imagerie Adaptative Diagnostique et Interventionnelle (IADI), and Université de Lorraine (UL)-Institut National de la Santé et de la Recherche Médicale (INSERM)
- Subjects
Models, Anatomic ,Aging ,Noninvasive imaging ,Anatomical structures ,Biology ,Sensitivity and Specificity ,Fetal Development ,Nuclear magnetic resonance spectrometer ,Imaging, Three-Dimensional ,Nuclear magnetic resonance ,Pregnancy ,Magnetic resonance microscopy ,Micro-MRI ,Carnegie stages ,medicine ,Humans ,Human embryo ,medicine.diagnostic_test ,Reproducibility of Results ,Developmental Anatomy ,Magnetic resonance histology ,Magnetic resonance imaging ,General Medicine ,Anatomy ,Embryo, Mammalian ,Magnetic Resonance Imaging ,3. Good health ,Pregnancy Trimester, First ,Developmental anatomy ,Female ,Human descriptive embryology ,Biological imaging ,[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology ,Developmental Biology - Abstract
Background and aim Technological advances in the field of biological imaging now allow multi-modal studies of human embryo anatomy. The aim of this study was to assess the high magnetic field μMRI feasibility in the study of small human embryos (less than 21 mm crown-rump) as a new tool for the study of human descriptive embryology and to determine better sequence characteristics to obtain higher spatial resolution and higher signal/noise ratio. Methods Morphological study of four human embryos belonging to the historical collection of the Department of Anatomy in the Faculty of Medicine of Reims was undertaken by μMRI. These embryos had, successively, crown-rump lengths of 3 mm (Carnegie Stage, CS 10), 12 mm (CS 16), 17 mm (CS 18) and 21 mm (CS 20). Acquisition of images was performed using a vertical nuclear magnetic resonance spectrometer, a Bruker Avance III, 500 MHz, 11.7 T equipped for imaging. Results All images were acquired using 2D (transverse, sagittal and coronal) and 3D sequences, either T 1 -weighted or T 2 -weighted. Spatial resolution between 24 and 70 μm/pixel allowed clear visualization of all anatomical structures of the embryos. Conclusion The study of human embryos μMRI has already been reported in the literature and a few atlases exist for educational purposes. However, to our knowledge, descriptive or morphological studies of human developmental anatomy based on data collected these few μMRI studies of human embryos are rare. This morphological noninvasive imaging method coupled with other techniques already reported seems to offer new perspectives to descriptive studies of human embryology.
- Published
- 2014
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