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Background: Osteoarticular infections in adults and children are a significant cause of elevated morbidity and may lead to restrictive mobility of various stages. Objective: Isolation and determination of the occurrence, pathomorphological and antibiotic susceptibility patterns of isolated microorganisms from the patients with osteoarticular infections. Methods: This research work was carried out at the Institute of Molecular Biology and Biotechnology (IMBB), The University of Lahore (UOL). Pus specimens (n = 120) were gathered from patients of osteoarticular infections. Bacterial isolates were purified and identified biochemically. Antibiotic resistance of the bacterial isolates was investigated by the criterion set by Clinical and Laboratory Standards Institute (CLSI). All experiments were run in triplicate using randomized study design. The mean, standard error and standard deviation values were determined using SPSS (v. 23.0). Results: Out of 120 samples, 111 isolated samples (93%) were tested positive for total viable count. The isolated bacterial species were observed to be Streptococcus pyogenes, Staphylococcus hemolyticus, S. aureus, Pseudomonas aeruginosa, Escherichia coli, Micrococcus sp., Serratia sp., Klebsiella sp., Enterobacter sp., and Proteus sp. Antibiogram results also yielded S. pyogenes and S. hemolyticus to be erythromycin resistant, while S. aureus was vancomycin resistant. E. coli and Klebsiella sp. were found to be resistant to tobramycin while Proteus and Enterobacter sp. were both sensitive to it. Conclusion: P. aeruginosa, E. coli, and S. aureus were prevalent in all groups of age, while Micrococcus and Serratia sp. were common in 16-55 years. Patient hygiene, immune health and the course of medications are all factors that should be kept in consideration while treating the disease. [ABSTRACT FROM AUTHOR]

Background: Infection of the bone or bone marrow simply means osteomyelitis. The classification is based on the type of bacteria, route, duration, and anatomical location of the involved area. Commonly it occurs due to exposure of the bone to the local infection. It usually requires prolong treatment lasting from weeks to months and may requires surgical intervention. Severe cases may lead to the loss of a limb. Objective: To determine the most common causative organism of osteoarticular infection in Department of Orthopaedics Surgery and Traumatology, Mayo Hospital, Lahore. Methodology: This longitudinal interventional study was conducted at DOST, Mayo Hospital Lahore for period of two years. Total 200 patients with osteoarticular infections were included. Informed consent and demographic detail was obtained. Patients were followed- up in OPD for 24 weeks post-operatively to assess the outcome of the procedure. Results: There were 180 (90%) males and 20 (10%) females. There were 39 (19.5%) patients who had upper limb infection, 147 (73.5%) patients with lower limb infections and 14 (7%) patients presented with septic arthritis of lower limb. Detailed clinical history of the patients shows that there were 186 (93%) patients who had previous history of trauma while 14 (7%) did not report trauma. Final outcome was assessed through a scoring system by Jaberi FM. After assessment, it was revealed that Culture sensitivity was positive in 188 (61.5%) patients at 1st week, at 6th week 123 (61.5%) patients had positive culture sensitivity and at 16th week, 52 (26%) cases had positive culture sensitivity. Causative organism were also given in the table Staph Aureus was the organism which cause infection in 96% of the patients. Conclusion: Most cases are post-traumatic and Staph Aureus is the most common causative organism found. Chronic Osteomyelitis is prevalent than acute one. [ABSTRACT FROM AUTHOR]