The objective of this study was to isolate, identify, and investigate lactic acid bacteria (LAB) present in tomato pomace. In total, we isolated 108 LAB strains and subsequently identified 16 species (87.5 % homofermentative LAB). Among these, the isolation of Lactobacillus harbinensis, L. manihotivorans, L. helveticus, L. camelliae, L. pontis, L. amylovorus, L. hilgardii, L. panis, L. vaginalis, and L. rapi from vegetable samples is reported for the first time. This is the first report to describe the diversity and natural populations of LAB associated with tomato pomace. The physiological and biochemical properties of the isolates could provide a platform for the future design of LAB inoculants aimed at improving silage quality.