1. Structure/activity relationships in basic FGF
- Author
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Yakov Gluzman, Andrew P. Seddon, Thomas Muller, Douglas C. Armellino, Imre Kovesdi, Peter Bohlen, and Mildred Decker
- Subjects
Binding Sites ,Chemistry ,Heparin ,General Neuroscience ,Mutant ,Biological activity ,Receptors, Cell Surface ,Pronase ,In Vitro Techniques ,Fibroblast growth factor ,Receptors, Fibroblast Growth Factor ,General Biochemistry, Genetics and Molecular Biology ,In vitro ,Peptide Fragments ,Recombinant Proteins ,Cell biology ,Structure-Activity Relationship ,History and Philosophy of Science ,Structure–activity relationship ,Humans ,Fibroblast Growth Factor 2 ,Disulfides ,Binding site ,Mitogens ,Cysteine - Abstract
Although the FGFs have been subject to extensive biological studies, only limited progress has been made so far in determining the critical elements of structure-activity relationships in the FGFs. Among the recognized structural elements with potential to affect the biological activity of FGFs are the cysteine residues, and the heparin- and receptor-binding domains. These features have been studied using a variety of experimental approaches, but the available data are inconclusive. For example, ambiguity regarding the presence of a disulfide structure in FGFs was not resolved until the availability of x-ray crystal structure data. Furthermore, the functionally important heparin- and receptor-binding domains have been poorly characterized, with some interpretations being controversial. In this report, we describe a novel fragment of basic FGF (bFGF) with high biological activity [Ser78,96-bFGF(70-153)]. This fragment was generated by pronase treatment of heparin-bound recombinant Glu3,5Ser78,96-bFGF mutant and is active in vitro at an ED50 of about 100 ng/ml. The structure of the fragment and the manner by which it was generated provide additional insight into important aspects of structure-activity relationships in FGFs. Specifically, we conclude that (a) the cysteines in our bFGF mutant do not form a disulfide bond, (b) the high-affinity heparin binding of bFGF critically depends on an intact 3-dimensional structure of the growth factor rather than on specific heparin-binding sequence domains, and (c) the bFGF sequence between residues 70 and 122 is important for high biological activity.
- Published
- 1991