1. Mechanism of PNA transport to the nuclear compartment.
- Author
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Stankova L, Ziemba AJ, Zhilina ZV, and Ebbinghaus SW
- Subjects
- Base Sequence, Cell Line, Chromosomes metabolism, Fluorescent Dyes, Guanosine 5'-O-(3-Thiotriphosphate) pharmacology, Humans, Nuclear Pore, Promoter Regions, Genetic, Receptor, ErbB-2 genetics, Active Transport, Cell Nucleus, Cell Nucleus metabolism, Peptide Nucleic Acids pharmacokinetics
- Abstract
We evaluated the nuclear uptake of fluorescently labeled peptide nucleic acids and measured the binding of unlabeled peptide nucleic acids (PNAs) to the endogenous HER-2/neu promotor in digitonin-permeabilized SK-BR-3 cells. Fluorescently labeled PNAs readily enter the nucleus of digitonin-permeabilized cells, and binding to the chromosomal target sequence was detected with a bis-PNA. Nuclear uptake and target sequence binding were inhibited by N-ethylmaleimide (NEM) and GTPgammaS. We conclude that PNAs are transported into the nucleus through an energy-dependent process involving the nuclear pore complex.
- Published
- 2006
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