1. Selection of Thioaptamers for Diagnostics and Therapeutics
- Author
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Bruce A. Luxon, David E. Volk, David W.C. Beasley, Alan D.T. Barrett, He Wang, Lee Lomas, Norbert K. Herzog, Xu Zhao, David G. Gorenstein, Judith F. Aronson, Xianbin Yang, and James F. Leary
- Subjects
Proteomics ,Genetics ,West Nile virus ,General Neuroscience ,Aptamer ,Drug Evaluation, Preclinical ,Protein Array Analysis ,Computational biology ,Aptamers, Nucleotide ,Thionucleotides ,Biology ,medicine.disease_cause ,General Biochemistry, Genetics and Molecular Biology ,Mice ,History and Philosophy of Science ,Proteome ,medicine ,Animals ,Humans ,West Nile Fever ,Selection (genetic algorithm) ,Transcription Factors - Abstract
Thioaptamers offer advantages over normal phosphate ester backbone aptamers due to their enhanced affinity, specificity, and higher stability, largely due to the properties of the sulfur backbone modifications. Over the past several years, in vitro thioaptamer selection and bead-based thioaptamer selection techniques have been developed in our laboratory. Furthermore, several thioaptamers targeting specific proteins such as transcription factor NF-kappaB and AP-1 proteins have been identified. Selected thioaptamers have been shown diagnostic promise in proteome screens. Moreover, some promising thioaptamers have been shown in preliminary animal therapeutic dosing to increase survival in animal models of infection with West Nile virus.
- Published
- 2006
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