Mycoplasma dispar is an overlooked pathogen often involved in bovine respiratory disease (BRD), which affects cattle around the world. BRD results in lost production and high treatment and prevention costs. Additionally, chronic therapies with multiple antimicrobials may lead to antimicrobial resistance. Data on antimicrobial susceptibility to M. dispar is limited so minimum inhibitory concentrations (MIC) of a range of antimicrobials routinely used in BRD were evaluated using a broth microdilution technique for 41 M. dispar isolates collected in Italy between 2011&ndash, 2019. While all isolates had low MIC values for florfenicol (<, 1 &mu, g/mL), many showed high MIC values for erythromycin (MIC90 &ge, 8 &mu, g/mL). Tilmicosin MIC values were higher (MIC50 = 32 &mu, g/mL) than those for tylosin (MIC50 = 0.25 &mu, g/mL). Seven isolates had high MIC values for lincomycin, tilmicosin and tylosin (&ge, 32 &mu, g/mL). More, alarmingly, results showed more than half the strains had high MICs for enrofloxacin, a member of the fluoroquinolone class considered critically important in human health. A time-dependent progressive drift of enrofloxacin MICs towards high-concentration values was observed, indicative of an on-going selection process among the isolates.