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The ESKAPE group ( Enterococcus faecium , Staphylococcus aureus , Klebsiella Pneumoniae , Acinetobacter baumannii , Pseudomonas aeruginosa , Enterobacter spp.) is a group of bacteria very difficult to treat due to their high ability to acquire resistance to antibiotics and are the main cause of nosocomial infections worldwide, posing a threat to global public health. Nosocomial infections with MDR bacteria are found mainly in Intensive Care Units, due to the multitude of maneuvers and invasive medical devices used, the prolonged antibiotic treatments, the serious general condition of these critical patients, and the prolonged duration of hospitalization., Materials and Methods: During a period of one year, from January 2023 to December 2023, this cross-sectional study was conducted on patients diagnosed with sepsis admitted to the Intensive Care Unit of the Sibiu County Emergency Clinical Hospital. Samples taken were tracheal aspirate, catheter tip, pharyngeal exudate, wound secretion, urine culture, blood culture, and peritoneal fluid., Results: The most common bacteria isolated from patients admitted to our Intensive Care Unit was Klebsiella pneumoniae , followed by Acinetobacter baumanii and Pseudomonas aeruginosa . Gram-positive cocci ( Enterococcus faecium and Staphilococcus aureus ) were rarely isolated. Most of the bacteria isolated were MDR bacteria., Conclusions: The rise of antibiotic and antimicrobial resistance among strains in the nosocomial environment and especially in Intensive Care Units raises serious concerns about limited treatment options.