Search

Your search keyword '"Tadao, Ito"' showing total 2 results
Start Over Author "Tadao, Ito" Journal anticancer research
2 results on '"Tadao, Ito"'

1. Dextran sulfate suppresses cell adhesion and cell cycle progression of melanoma cells

Author

Tsuyoshi, Takagi, Chouhei, Sakakura, Shuichi, Kin, Yuen, Nakase, Kenichiro, Fukuda, Katsumi, Shimomura, Tadao, Ito, Junshin, Fujiyama, Junya, Yamasaki, Hiroyuki, Tsujimoto, Yasushi, Okazaki, Yoshihide, Hayashizaki, Hisakazu, Yamagishi, and Akeo, Hagiwara

Subjects
Mice, Blotting, Western, Cell Cycle, Dextran Sulfate, Cell Adhesion, Melanoma, Experimental, Animals, Blotting, Northern, Flow Cytometry, Oligonucleotide Array Sequence Analysis
Abstract

We have reported that dextran sulfate is a possible candidate for an antimetastatic drug because it inhibits cell adhesion. It has been demonstrated that dextran sulfate can detach cancer cells adhering to the bottom of plastic flasks, and that the detached cells do not readhere or proliferate. In this study, we investigated the effects of dextran sulfate on cancer cells, focusing on cell cycle regulators as well as cell adhesion molecules. The effects of dextran sulfate on the cell cycle were examined by flow cytometry, and changes in gene expression caused by dextran sulfate were analyzed by cDNA microarray to identify changes in adhesion and cell cycle genes. By flow cytometry, treatment with dextran sulfate increased the percentage of cells in the G1/G0-phase, and decreased those in the S- and G2/M-phases. Analysis by cDNA microarray revealed decreased expression of several genes essential for progression of the G1- and S-phases. The expression of the adhesion factors involved in metastases was also suppressed. Furthermore, we confirmed these changes in the gene expression by Northern and Western blotting. Our results indicate that dextran sulfate suppresses cell adhesion and cell cycle progression, both of which are essential for metastasis, suggesting that dextran sulfate could be used as an antimetastatic agent.

Published
2005

2. Local administration of methotrexate bound to activated carbon particles (MTX-CH) for treating cancers in mice

Author

Tadao, Ito, Akeo, Hagiwara, Tsuyoshi, Takagi, Junshin, Fujiyama, Yoshinobu, Sonoyama, Katsumi, Shimomura, Manabu, Takemura, Atsushi, Toma, Shuichi, Kin, Yuen, Nakase, and Hisakazu, Yamagishi

Subjects
Male, Antimetabolites, Antineoplastic, Drug Carriers, Mice, Inbred BALB C, Povidone, Injections, Intralesional, Drug Administration Schedule, Solutions, Mice, Methotrexate, Charcoal, Colonic Neoplasms, Tumor Cells, Cultured, Animals, Drug Screening Assays, Antitumor, Particle Size, Neoplasm Transplantation
Abstract

A new dosage formulation of methotrexate (MTX-CH) was developed to control cancer growth by local administration.BALB/c mice received a subcutaneous inoculation with transplantable Colon 26 cancer cells on the back. When cancerous tumor became 7 mm in diameter, MTX-CH or MTX aqueous solution (MTX-sol) was injected into the tumor. The MTX concentration in the tumor was compared between the MTX-CH group and the MTX-sol group. The tumor growth was assessed in single or repeated local administration experiments.The MTX concentrations were significantly higher for longer periods in the MTX-CH group than those in the MTX-sol group. Repeated MTX-CH administration was significantly more effective for suppressing the tumor growth compared with repeated MTX-sol administration.MTX-CH is superior to MTX-sol in controlling the tumor growth by local administration because of its long-acting effect.

Published
2003

Catalog

Books, media, physical & digital resources
See catalog results