1. Effect of Ertapenem Protein Binding on Killing of Bacteria
- Author
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Emily C. Ferguson, David E. Nix, and Kathryn R. Matthias
- Subjects
Ertapenem ,Staphylococcus aureus ,Lactams ,medicine.drug_class ,Antibiotics ,Microbial Sensitivity Tests ,beta-Lactams ,medicine.disease_cause ,Staphylococcal infections ,Microbiology ,chemistry.chemical_compound ,Enterobacter cloacae ,medicine ,Humans ,Pharmacology (medical) ,Chromatography, High Pressure Liquid ,Antibacterial agent ,Pharmacology ,Bacteria ,biology ,Enterobacteriaceae Infections ,Staphylococcal Infections ,biology.organism_classification ,medicine.disease ,Antimicrobial ,Kinetics ,Infectious Diseases ,chemistry ,Regression Analysis ,Indicators and Reagents ,Spectrophotometry, Ultraviolet ,Protein Binding - Abstract
The effect of protein binding on the antimicrobial activity of ertapenem was evaluated using the bacterial kill rate and concentration-response studies. Various proportions of human serum were utilized to determine the total and free-drug concentrations using a validated high-performance liquid chromatography assay. The MICs and kill curves were determined for test isolates of Enterobacter cloacae and Staphylococcus aureus at various percentages of human serum. The killing of bacteria was analyzed in relation to the free and total concentrations of ertapenem at various proportions of human serum. It was determined that unbound ertapenem was responsible for the antimicrobial activity against the test isolates.
- Published
- 2004
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