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Your search keyword '"Wiederhold, N P"' showing total 3 results
Start Over Author "Wiederhold, N P" Journal antimicrobial agents and chemotherapy
3 results on '"Wiederhold, N P"'

1. International Evaluation of MIC Distributions and Epidemiological Cutoff Value (ECV) Definitions for Fusarium Species Identified by Molecular Methods for the CLSI Broth Microdilution Method

Author

Espinel-Ingroff, A., primary, Colombo, A. L., additional, Cordoba, S., additional, Dufresne, P. J., additional, Fuller, J., additional, Ghannoum, M., additional, Gonzalez, G. M., additional, Guarro, J., additional, Kidd, S. E., additional, Meis, J. F., additional, Melhem, T. M. S. C., additional, Pelaez, T., additional, Pfaller, M. A., additional, Szeszs, M. W., additional, Takahaschi, J. P., additional, Tortorano, A. M., additional, Wiederhold, N. P., additional, and Turnidge, J., additional

Published
2016
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2. International Evaluation of MIC Distributions and Epidemiological Cutoff Value (ECV) Definitions for FusariumSpecies Identified by Molecular Methods for the CLSI Broth Microdilution Method

Author

Espinel-Ingroff, A., Colombo, A. L., Cordoba, S., Dufresne, P. J., Fuller, J., Ghannoum, M., Gonzalez, G. M., Guarro, J., Kidd, S. E., Meis, J. F., Melhem, T. M. S. C., Pelaez, T., Pfaller, M. A., Szeszs, M. W., Takahaschi, J. P., Tortorano, A. M., Wiederhold, N. P., and Turnidge, J.

Abstract

ABSTRACTThe CLSI epidemiological cutoff values (ECVs) of antifungal agents are available for various Candidaspp., Aspergillusspp., and the Mucorales. However, those categorical endpoints have not been established for Fusariumspp., mostly due to the difficulties associated with collecting sufficient CLSI MICs for clinical isolates identified according to the currently recommended molecular DNA-PCR-based identification methodologies. CLSI MIC distributions were established for 53 Fusarium dimerumspecies complex (SC), 10 F. fujikuroi, 82 F. proliferatum, 20 F. incarnatum-F. equisetiSC, 226 F. oxysporumSC, 608 F. solaniSC, and 151 F. verticillioidesisolates originating in 17 laboratories (in Argentina, Australia, Brazil, Canada, Europe, Mexico, and the United States). According to the CLSI guidelines for ECV setting, ECVs encompassing ≥97.5% of pooled statistically modeled MIC distributions were as follows: for amphotericin B, 4 μg/ml (F. verticillioides) and 8 μg/ml (F. oxysporumSC and F. solaniSC); for posaconazole, 2 μg/ml (F. verticillioides), 8 μg/ml (F. oxysporumSC), and 32 μg/ml (F. solaniSC); for voriconazole, 4 μg/ml (F. verticillioides), 16 μg/ml (F. oxysporumSC), and 32 μg/ml (F. solaniSC); and for itraconazole, 32 μg/ml (F. oxysporumSC and F. solaniSC). Insufficient data precluded ECV definition for the other species. Although these ECVs could aid in detecting non-wild-type isolates with reduced susceptibility to the agents evaluated, the relationship between molecular mechanisms of resistance (gene mutations) and MICs still needs to be investigated for Fusariumspp.

Published
2015
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3. International Evaluation of MIC Distributions and Epidemiological Cutoff Value (ECV) Definitions for Fusarium Species Identified by Molecular Methods for the CLSI Broth Microdilution Method.

Author

Espinel-Ingroff A, Colombo AL, Cordoba S, Dufresne PJ, Fuller J, Ghannoum M, Gonzalez GM, Guarro J, Kidd SE, Meis JF, Melhem TM, Pelaez T, Pfaller MA, Szeszs MW, Takahaschi JP, Tortorano AM, Wiederhold NP, and Turnidge J

Subjects
Americas, Drug Resistance, Multiple, Fungal, Europe, Fusarium genetics, Fusarium isolation & purification, Humans, Polymerase Chain Reaction methods, Antifungal Agents pharmacology, Fusarium drug effects, Microbial Sensitivity Tests methods
Abstract

The CLSI epidemiological cutoff values (ECVs) of antifungal agents are available for various Candida spp., Aspergillus spp., and the Mucorales. However, those categorical endpoints have not been established for Fusarium spp., mostly due to the difficulties associated with collecting sufficient CLSI MICs for clinical isolates identified according to the currently recommended molecular DNA-PCR-based identification methodologies. CLSI MIC distributions were established for 53 Fusarium dimerum species complex (SC), 10 F. fujikuroi, 82 F. proliferatum, 20 F. incarnatum-F. equiseti SC, 226 F. oxysporum SC, 608 F. solani SC, and 151 F. verticillioides isolates originating in 17 laboratories (in Argentina, Australia, Brazil, Canada, Europe, Mexico, and the United States). According to the CLSI guidelines for ECV setting, ECVs encompassing ≥97.5% of pooled statistically modeled MIC distributions were as follows: for amphotericin B, 4 μg/ml (F. verticillioides) and 8 μg/ml (F. oxysporum SC and F. solani SC); for posaconazole, 2 μg/ml (F. verticillioides), 8 μg/ml (F. oxysporum SC), and 32 μg/ml (F. solani SC); for voriconazole, 4 μg/ml (F. verticillioides), 16 μg/ml (F. oxysporum SC), and 32 μg/ml (F. solani SC); and for itraconazole, 32 μg/ml (F. oxysporum SC and F. solani SC). Insufficient data precluded ECV definition for the other species. Although these ECVs could aid in detecting non-wild-type isolates with reduced susceptibility to the agents evaluated, the relationship between molecular mechanisms of resistance (gene mutations) and MICs still needs to be investigated for Fusarium spp., (Copyright © 2016, American Society for Microbiology. All Rights Reserved.)

Published
2015
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