1. Development of a rapid antiviral screening assay based on eGFP reporter virus of Mayaro virus
- Author
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Hong-Qing Zhang, Bo Zhang, Ana Carolina Bernardes Terzian, Xiao-Dan Li, Ya-Nan Zhang, Ana Carolina Gomes Jardim, Cheng-Lin Deng, Zhen Wang, Jia-Qi Li, and Maurício Lacerda Nogueira
- Subjects
0301 basic medicine ,Recombinant Fusion Proteins ,viruses ,Green Fluorescent Proteins ,030106 microbiology ,Drug Evaluation, Preclinical ,Alphavirus ,Genome, Viral ,Virus Replication ,medicine.disease_cause ,Antiviral Agents ,Genomic Instability ,Virus ,Cell Line ,Zika virus ,Dengue fever ,03 medical and health sciences ,chemistry.chemical_compound ,Genes, Reporter ,Cricetinae ,Virology ,medicine ,Animals ,Chikungunya ,Pharmacology ,biology ,Ribavirin ,virus diseases ,biology.organism_classification ,medicine.disease ,Reverse genetics ,Culicidae ,030104 developmental biology ,chemistry ,Viral replication - Abstract
Mayaro virus (MAYV) is a neglected mosquito-borne alphavirus that causes illness similar to Chikungunya (CHIKV), Dengue (DENV) and Zika virus (ZIKV). Currently, there is no specific treatment or vaccine against MAYV infection. To develop an efficient antiviral screening assay for MAYV, we constructed the infectious clones of MAYV strain BeAr 20290 and its eGFP reporter virus. The reporter virus exhibited high replication capacity indistinguishable with the wild type MAYV, and was genetically stable within at least five rounds of passages in BHK-21 cell. The expression of eGFP correlated well with the viral replication. Using the known inhibitor ribavirin, we confirmed that the MAYV-eGFP reporter virus could be used for antiviral screening to identify the specific inhibitors against MAYV. Using the MAYV-eGFP based antiviral assay, we found that the compound 6-Azauridine which had antiviral activity against CHIKV and SFV, showed a significant inhibitory effect on MAYV replication.
- Published
- 2019
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